diff --git a/assays/qRT-PCR/protocols/qRT-PCR.md b/assays/qRT-PCR/protocols/qRT-PCR.md
new file mode 100644
index 0000000000000000000000000000000000000000..f6147b3bf2f78b7f0e3d77635386660e7310cbb2
--- /dev/null
+++ b/assays/qRT-PCR/protocols/qRT-PCR.md
@@ -0,0 +1,2 @@
+## qRT-PCR
+To quantify fungal colonization and *PR10* gene expression, RNA extraction, cDNA generation and RT-PCR were performed as previously described (Sarkar et al., 2019).
\ No newline at end of file
diff --git a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/SerendipitaIndicaSerendipitaVermiferaAndBipolarisSorokiniana.md b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/SerendipitaIndicaSerendipitaVermiferaAndBipolarisSorokiniana.md
index da1b0f289d7a85c16111beab4229d455db6c17f0..56f170b4dd67feaf4f54ccddffcc85b960c63e6a 100644
--- a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/SerendipitaIndicaSerendipitaVermiferaAndBipolarisSorokiniana.md
+++ b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/SerendipitaIndicaSerendipitaVermiferaAndBipolarisSorokiniana.md
@@ -1,2 +1,2 @@
 ## Serendipita indica, Serendipita vermifera and Bipolaris sorokiniana
-The growth conditions for barley, *S. indica* (DSM11827, Si), *S. vermifera* (MAFF305830, Sv) and *B. sorokiniana* (ND90Pr, Bs) and the preparation of fungal suspensions for plant inoculation have been described previously.41,75 Four-day-old barley seedlings were transferred to sterile jars on 1/10 PNM (pH 5.7) and inoculated with 3 mL of either sterile water as control, *Si* chlamydospores (500,000 spores·mL-1), *Sv* mycelium (1 g per 50 mL), or *Bs* conidia (5,000 spores·mL-1). Plants were grown on a day/night cycle of 16/8 h at 22/18 °C and 60% humidity under a light intensity of 108 μmol·m−2·s−1. Plant roots were harvested six days post inoculation (dpi), washed thoroughly to remove extraradical fungal hyphae, and frozen in liquid nitrogen. Four barley plants were pooled per biological replicate. To quantify fungal colonization and *PR10* gene expression, RNA extraction, cDNA generation and RT-PCR were performed as previously described.41
\ No newline at end of file
+The growth conditions for barley, *S. indica* (DSM11827, Si), *S. vermifera* (MAFF305830, Sv) and *B. sorokiniana* (ND90Pr, Bs) and the preparation of fungal suspensions for plant inoculation have been described previously.41,75 Four-day-old barley seedlings were transferred to sterile jars on 1/10 PNM (pH 5.7) and inoculated with 3 mL of either sterile water as control, *Si* chlamydospores (500,000 spores·mL-1), *Sv* mycelium (1 g per 50 mL), or *Bs* conidia (5,000 spores·mL-1). Plants were grown on a day/night cycle of 16/8 h at 22/18 °C and 60% humidity under a light intensity of 108 μmol·m−2·s−1. Plant roots were harvested six days post inoculation (dpi), washed thoroughly to remove extraradical fungal hyphae, and frozen in liquid nitrogen. Four barley plants were pooled per biological replicate. 
\ No newline at end of file