diff --git a/README.md b/README.md index 5518a0251b21ee0dcd417ba402973c2999a268cd..128f53241c50183ecb3a98c111f258126817341a 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,6 @@ # A GH81-type β-glucan-binding protein enhances colonization by mutualistic fungi in barley ## Original publication Wanke, A., van Boerdonk, S., Mahdi, L. K., Wawra, S., Neidert, M., Chandrasekar, B., Saake, P., Saur, I. M. L., Derbyshire, P., Holton, N., Menke, F. L. H., Brands, M., Pauly, M., Acosta, I. F., Zipfel, C., & Zuccaro, A. (2023). A GH81-type β-glucan-binding protein enhances colonization by mutualistic fungi in barley. Current biology : CB, 33(23), 5071–5084.e7. https://doi.org/10.1016/j.cub.2023.10.048 + ## Copyright Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved. diff --git a/assays/EnzymaticCarbohydrateDigestionAndTLC/isa.assay.xlsx b/assays/EnzymaticCarbohydrateDigestionAndTLC/isa.assay.xlsx index 0e78952e1ea29bb0f26d53fdc03fd01c8265a513..845bbe7a5e59b3696034c999c3ca3a367618cb25 100644 Binary files a/assays/EnzymaticCarbohydrateDigestionAndTLC/isa.assay.xlsx and b/assays/EnzymaticCarbohydrateDigestionAndTLC/isa.assay.xlsx differ diff --git a/assays/MALDI-TOFAnalysis/isa.assay.xlsx b/assays/MALDI-TOFAnalysis/isa.assay.xlsx index 2156bb9e0230d180536c193c5b5f7df8f722e092..3bac43be62341c10b4a6e2e257834200a7cbef44 100644 Binary files a/assays/MALDI-TOFAnalysis/isa.assay.xlsx and b/assays/MALDI-TOFAnalysis/isa.assay.xlsx differ diff --git a/assays/MS-MSAnalysisOfThePull-downProteins/isa.assay.xlsx b/assays/MS-MSAnalysisOfThePull-downProteins/isa.assay.xlsx index efa479c207584510d92a0bc9d6b6b87a823b68ec..22acf68e3d370beeeb36b272f16a40a32975069a 100644 Binary files a/assays/MS-MSAnalysisOfThePull-downProteins/isa.assay.xlsx and b/assays/MS-MSAnalysisOfThePull-downProteins/isa.assay.xlsx differ diff --git a/assays/OxidativeBurstAssay/isa.assay.xlsx b/assays/OxidativeBurstAssay/isa.assay.xlsx index c0c106d40bff98d5f139e72dd9d812f371d7cdca..aec248b1d93b6487e656d3a3507cb7c3744555fb 100644 Binary files a/assays/OxidativeBurstAssay/isa.assay.xlsx and b/assays/OxidativeBurstAssay/isa.assay.xlsx differ diff --git a/assays/QuantificationAndStatisticalAnalyses/isa.assay.xlsx b/assays/QuantificationAndStatisticalAnalyses/isa.assay.xlsx index 866652f6c15fed533f794d87b5983219e91a89d4..ef63a2b8c5246a36ee185d71e6465f81e95960c0 100644 Binary files a/assays/QuantificationAndStatisticalAnalyses/isa.assay.xlsx and b/assays/QuantificationAndStatisticalAnalyses/isa.assay.xlsx differ diff --git a/assays/qRT-PCR/README.md b/assays/qRT-PCR/README.md new file mode 100644 index 0000000000000000000000000000000000000000..58f00dda71411dbc61d4b89353842393ecf4b3e8 --- /dev/null +++ b/assays/qRT-PCR/README.md @@ -0,0 +1,9 @@ +<img src=dataset\grp2_lrg.jpg width=60%> + +### Figure 2 caption + +**Figure 2.** Mutation of barley GBP1 GBP2 decreases colonization by the fungal pathogens B. sorokiniana and B. hordei +To test the impact of GBP mutation on the compatibility of pathogenic fungi, barley roots and shoots were inoculated with B. sorokiniana (left graph) and B. hordei (right graph), respectively. Barley control and gbp mutant lines were inoculated with B. sorokiniana and grown in jars under axenic conditions for 7 days (Figure S3C). To assess the degree of root colonization, the expression of B. sorokiniana housekeeping gene BsTEF was quantified by RT-PCR and normalized to the barley housekeeping gene ubiquitin HvUBI. Barley leaves colonized by B. hordei were analyzed for penetration success using bright-field microscopy (Figure S3D). Boxplot represents quantification of B. hordei penetration success at 48 h. Boxplot elements in this figure: center line, median; box limits, upper and lower quartiles; whiskers, 1.5× interquartile range. Data points from independent experiments are indicated by data point shape. Different letters represent statistically significant differences based on one-way ANOVA and Tukey’s post hoc test (significance threshold: p ≤ 0.05). + +### Figure 2 source +https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#fx1 \ No newline at end of file diff --git a/assays/qRT-PCR/dataset/.gitkeep b/assays/qRT-PCR/dataset/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/qRT-PCR/dataset/gr2_lrg.jpg b/assays/qRT-PCR/dataset/gr2_lrg.jpg new file mode 100644 index 0000000000000000000000000000000000000000..ee5339b16733253d6539107da770fa19f7e376b1 --- /dev/null +++ b/assays/qRT-PCR/dataset/gr2_lrg.jpg @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:887857253a48deb938a9d724b1f24071577cdeed4326f113a170358212065bd6 +size 279811 diff --git a/assays/qRT-PCR/isa.assay.xlsx b/assays/qRT-PCR/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..08367b357959ea6e5022e6e9fb16a81cef586ca0 Binary files /dev/null and b/assays/qRT-PCR/isa.assay.xlsx differ diff --git a/assays/qRT-PCR/protocols/.gitkeep b/assays/qRT-PCR/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/isa.investigation.xlsx b/isa.investigation.xlsx index 1293f5535cdc09e7d75306a408f6ab6a5045ec14..4cda9ba889df9d742c9fca80fd79c905bb7bf0d0 100644 Binary files a/isa.investigation.xlsx and b/isa.investigation.xlsx differ diff --git a/studies/Barley/isa.study.xlsx b/studies/Barley/isa.study.xlsx index 42c210e52a09c155f61b113276ecb1d5d7bb7171..a4498d257f68a5bbb0d08692fc47d7b1851add1c 100644 Binary files a/studies/Barley/isa.study.xlsx and b/studies/Barley/isa.study.xlsx differ diff --git a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.study.xlsx b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.study.xlsx index 5219cde0b8fe5b7ada6fc6bdbec3802e05a4f95e..bf74f8504d45c8113f7ecc934e6739debf3b2089 100644 Binary files a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.study.xlsx and b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.study.xlsx differ diff --git a/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx b/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx index 5fb9c065d8822c1e956388c80124f881ba06e9e6..54ca6e9a7a8069f70af054f835a06c50e8a331dc 100644 Binary files a/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx and b/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx differ