diff --git a/isa.investigation.xlsx b/isa.investigation.xlsx index d3884114a75d1ef5fb883f63216abb77fee8cced..2b4b6b43572bb1a493e81348084a2688d136228d 100644 Binary files a/isa.investigation.xlsx and b/isa.investigation.xlsx differ diff --git a/studies/Barley/isa.study.xlsx b/studies/Barley/isa.study.xlsx index 888120128b6c17d33ef0193168ec03ea28bda33e..151360de7840a71925050dd580e33c33d13f3a7f 100644 Binary files a/studies/Barley/isa.study.xlsx and b/studies/Barley/isa.study.xlsx differ diff --git a/studies/PlantMaterialAndGrowthConditions/README.md b/studies/PlantMaterialAndGrowthConditions/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx b/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..ecc8bebe0726a8a60820d09c4cb0567f906e460e Binary files /dev/null and b/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx differ diff --git a/studies/PlantMaterialAndGrowthConditions/protocols/.gitkeep b/studies/PlantMaterialAndGrowthConditions/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/PlantMaterialAndGrowthConditions/protocols/HordeumVulgare.md b/studies/PlantMaterialAndGrowthConditions/protocols/HordeumVulgare.md new file mode 100644 index 0000000000000000000000000000000000000000..70b001d305bf1ed4a555192e35ff1c477402e46b --- /dev/null +++ b/studies/PlantMaterialAndGrowthConditions/protocols/HordeumVulgare.md @@ -0,0 +1,2 @@ +## Hordeum vulgare +All experiments, including the generation of CRISPR/Cas9 knock-out lines, were performed with the spring barley (*H. vulgare L.*) cv. Golden Promise Fast, an introgression line carrying the Ppd-H1 allele that confers fast flowering.42 From here on, we use “control†to name this non-mutagenized cultivar that carries normal copies of *GBP1* and *GBP2*. For ROS burst assays, barley seeds were surface sterilized with 6% sodium hypochlorite for 1 h and then washed extensively (5 × 30 mL sterile water). Seeds were germinated on wet filter paper at room temperature in the dark under sterile conditions for three days before transfer to sterile jars containing solid 1/10 plant nutrition medium (PNM), pH 5.7 and 0.4% Gelrite (Duchefa, Haarlem, the Netherlands).82 Seedlings were cultured for four days in a growth chamber under long-day conditions (day/night cycle of 16/8 h, 22 °C/18 °C, light intensity of 108 μmol·m−2·s−1). \ No newline at end of file diff --git a/studies/PlantMaterialAndGrowthConditions/resources/.gitkeep b/studies/PlantMaterialAndGrowthConditions/resources/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391