diff --git a/assays/CRISPRCas9-basedMutagenesis/isa.assay.xlsx b/assays/CRISPRCas9-basedMutagenesis/isa.assay.xlsx index a8eb7326c26d486c415a72b1ad67c74fa276a2e9..10c04fc19d26957702b82d3a9526097aa6808e3f 100644 Binary files a/assays/CRISPRCas9-basedMutagenesis/isa.assay.xlsx and b/assays/CRISPRCas9-basedMutagenesis/isa.assay.xlsx differ diff --git a/assays/EnzymaticCarbohydrateDigestionAndTLC/isa.assay.xlsx b/assays/EnzymaticCarbohydrateDigestionAndTLC/isa.assay.xlsx index 91e0aadae574afc892037eb1ec5226452a9e1d83..38d2376c4cde5346ecc8e5feef462254529f7199 100644 Binary files a/assays/EnzymaticCarbohydrateDigestionAndTLC/isa.assay.xlsx and b/assays/EnzymaticCarbohydrateDigestionAndTLC/isa.assay.xlsx differ diff --git a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/README.md b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/README.md similarity index 100% rename from studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/README.md rename to assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/README.md diff --git a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/.gitkeep b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/dataset/.gitkeep similarity index 100% rename from studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/.gitkeep rename to assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/dataset/.gitkeep diff --git a/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.assay.xlsx b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..d65dbca2cd4d9154cfb2d209ee574cbe28bcd42a Binary files /dev/null and b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.assay.xlsx differ diff --git a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/resources/.gitkeep b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/.gitkeep similarity index 100% rename from studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/resources/.gitkeep rename to assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/.gitkeep diff --git a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/BlumeriaHordei.md b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/BlumeriaHordei.md similarity index 99% rename from studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/BlumeriaHordei.md rename to assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/BlumeriaHordei.md index 3a3cfc41078744f89a5ae55c50f87a4be85467c2..f21c76b0bb2a81e4e97dfde7b007f142f15a4e50 100644 --- a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/BlumeriaHordei.md +++ b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/BlumeriaHordei.md @@ -1,2 +1,3 @@ ## Blumeria hordei + *Blumeria hordei* isolate K1 (Hinze et al., 1991) was maintained on intact plants of barley cv. Golden Promise grown in soil at 19 °C, 60% relative humidity and a photoperiod of 14 h with a light intensity of 100 μmol ·m-2 s-1. Control and *GBP* knock-out lines were grown in soil in a growth chamber (poly klima) at 19 °C, 60% relative humidity and a photoperiod of 14 h with a light intensity of 100 μmol ·m−2·s−1. Primary leaves of seven-day-old plants were cut and placed with the adaxial side down on 1% plant agar plates before gravity inoculation with *B. hordei* isolate K1 at a conidial density of about 20 conidia per mm. Leaves were harvested at 48 h and cleared in 70% ethanol before staining of the fungal structures with Coomassie brilliant blue solution (0.1% [w/v] Coomassie brilliant blue R-250 in 50% ethanol and 10% acetic acid) for 10-15 s. Bright field microscopy was used to assess secondary hyphae formation in 50 germinated conidia spores in the tip area and 50 germinated conidia in the middle of the leaf. At least six independent leaves were examined at 48 h after infection for fungal penetration success. \ No newline at end of file diff --git a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/RhizophagusIrregularis.md b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/RhizophagusIrregularis.md similarity index 99% rename from studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/RhizophagusIrregularis.md rename to assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/RhizophagusIrregularis.md index 1f8905e5ac8017941bd5e54a4d410d4827fa2420..9fe300d6100ddb7c176d601dd3f1e8191aca0f54 100644 --- a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/RhizophagusIrregularis.md +++ b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/RhizophagusIrregularis.md @@ -1,2 +1,3 @@ ## Rhizophagus irregularis + Barley control and mutant seeds were sterilized and germinated as described previously (Mahdi et al., 2022). Germinated seedlings were transferred to 9 × 9 cm pots containing autoclaved 1:1 silica sand:vermiculite mixture inoculated with 700 mg *R. irregularis* spore inoculum (10,000 spores·g-1 moist diatomaceous earth powder [50% water]) (Symplanta, Darmstadt, Germany). Approximately 500 mg of the inoculum was evenly mixed into the lower two-third substrate layer, further 200 mg were evenly sprinkled into a hole in the upper third of the substrate layer, into which the seedlings were transplanted. The seedlings were grown in the greenhouse and watered weekly with 30 mL of deionized water and tap water in a 1:1 ratio. Roots were harvested at 28 dpi and stored in 50% EtOH at 4 °C until staining. \ No newline at end of file diff --git a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/SerendipitaIndicaSerendipitaVermiferaAndBipolarisSorokiniana.md b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/SerendipitaIndicaSerendipitaVermiferaAndBipolarisSorokiniana.md similarity index 99% rename from studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/SerendipitaIndicaSerendipitaVermiferaAndBipolarisSorokiniana.md rename to assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/SerendipitaIndicaSerendipitaVermiferaAndBipolarisSorokiniana.md index f400c6714c507e4d9b1d52a06cf0c0aaeb2be0e2..edda83b9d70256a93c901b5b4140a336a6191b21 100644 --- a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/SerendipitaIndicaSerendipitaVermiferaAndBipolarisSorokiniana.md +++ b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/SerendipitaIndicaSerendipitaVermiferaAndBipolarisSorokiniana.md @@ -1,2 +1,3 @@ ## Serendipita indica, Serendipita vermifera and Bipolaris sorokiniana + The growth conditions for barley, *S. indica* (DSM11827, Si), *S. vermifera* (MAFF305830, Sv) and *B. sorokiniana* (ND90Pr, Bs) and the preparation of fungal suspensions for plant inoculation have been described previously (Sarkar et al., 2019; Mahdi et al., 2022). Four-day-old barley seedlings were transferred to sterile jars on 1/10 PNM (pH 5.7) and inoculated with 3 mL of either sterile water as control, *Si* chlamydospores (500,000 spores·mL-1), *Sv* mycelium (1 g per 50 mL), or *Bs* conidia (5,000 spores·mL-1). Plants were grown on a day/night cycle of 16/8 h at 22/18 °C and 60% humidity under a light intensity of 108 μmol·m−2·s−1. Plant roots were harvested six days post inoculation (dpi), washed thoroughly to remove extraradical fungal hyphae, and frozen in liquid nitrogen. 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