diff --git a/isa.investigation.xlsx b/isa.investigation.xlsx index 2b4b6b43572bb1a493e81348084a2688d136228d..416921f22966c41184cd124637f0097b93ad674d 100644 Binary files a/isa.investigation.xlsx and b/isa.investigation.xlsx differ diff --git a/studies/Barley/isa.study.xlsx b/studies/Barley/isa.study.xlsx index 151360de7840a71925050dd580e33c33d13f3a7f..c930a92fd7812501e2e6640d91490c3e634f2ee7 100644 Binary files a/studies/Barley/isa.study.xlsx and b/studies/Barley/isa.study.xlsx differ diff --git a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/README.md b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.study.xlsx b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.study.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..2a467d18442e307bd11444d56b01405d38620536 Binary files /dev/null and b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.study.xlsx differ diff --git a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/.gitkeep b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/SerendipitaIndicaSerendipitaVermiferaAndBipolarisSorokiniana.md b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/SerendipitaIndicaSerendipitaVermiferaAndBipolarisSorokiniana.md new file mode 100644 index 0000000000000000000000000000000000000000..da1b0f289d7a85c16111beab4229d455db6c17f0 --- /dev/null +++ b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/SerendipitaIndicaSerendipitaVermiferaAndBipolarisSorokiniana.md @@ -0,0 +1,2 @@ +## Serendipita indica, Serendipita vermifera and Bipolaris sorokiniana +The growth conditions for barley, *S. indica* (DSM11827, Si), *S. vermifera* (MAFF305830, Sv) and *B. sorokiniana* (ND90Pr, Bs) and the preparation of fungal suspensions for plant inoculation have been described previously.41,75 Four-day-old barley seedlings were transferred to sterile jars on 1/10 PNM (pH 5.7) and inoculated with 3 mL of either sterile water as control, *Si* chlamydospores (500,000 spores·mL-1), *Sv* mycelium (1 g per 50 mL), or *Bs* conidia (5,000 spores·mL-1). Plants were grown on a day/night cycle of 16/8 h at 22/18 °C and 60% humidity under a light intensity of 108 μmol·m−2·s−1. Plant roots were harvested six days post inoculation (dpi), washed thoroughly to remove extraradical fungal hyphae, and frozen in liquid nitrogen. Four barley plants were pooled per biological replicate. To quantify fungal colonization and *PR10* gene expression, RNA extraction, cDNA generation and RT-PCR were performed as previously described.41 \ No newline at end of file diff --git a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/resources/.gitkeep b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/resources/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx b/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx index ecc8bebe0726a8a60820d09c4cb0567f906e460e..9d0dbc1db37428384d176a380fcff6d11dcc7063 100644 Binary files a/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx and b/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx differ