From 93bb7745104386f11439fda950945183b0a9276c Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Mon, 8 Jan 2024 20:51:37 +0100
Subject: [PATCH] add supplementary figures captions in TLC assay

---
 .../README.md                                 | 34 ++++++++++++++++++-
 1 file changed, 33 insertions(+), 1 deletion(-)

diff --git a/assays/EnzymaticCarbohydrateDigestionAndTLC/README.md b/assays/EnzymaticCarbohydrateDigestionAndTLC/README.md
index 182b843..38e27fc 100644
--- a/assays/EnzymaticCarbohydrateDigestionAndTLC/README.md
+++ b/assays/EnzymaticCarbohydrateDigestionAndTLC/README.md
@@ -14,4 +14,36 @@ https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=h
 **Figure 4 A).** The digestion products resulting from the enzymatic breakdown of laminarin by GBP1 over time were analyzed using thin-layer chromatography (TLC). Untreated (UT) and GBP1E500A-treated laminarin (20 h) served as controls. Digestion was performed at 25°C.
 
 ### Figure 4 A) source
-https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#secsectitle0065
\ No newline at end of file
+https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#secsectitle0065
+
+<img src=dataset\FigS4A.jpg width=60%>
+
+### Supplementary Figure 4 A) caption
+
+**Supplementary Figure 4 A).** Laminarin (4 mg·mL−1) was digested with GBP1 (70 nM) for 10 min at different temperatures. Sample without enzymes (UT) was mock-digested at 80 °C. Products from GBP1-catalyzed laminarin digestion assays in (A) and (B) were separated by thin layer
+chromatography. Quantification of thin layer chromatographs in Figure 4C.
+
+### Supplementary Figure 4 A) source
+https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
+
+<img src=dataset\FigS4B.jpg width=60%>
+
+### Supplementary Figure 4 B) caption
+
+**Supplementary Figure 4 B).**  Laminarin (4 mg·mL−1) was digested with purified GBP1 (70 nM) in different buffers
+(10 mM, pH 5-9). Digestions were performed at 60 °C for 10 min. Products from GBP1-
+catalyzed laminarin digestion assays in (A) and (B) were separated by thin layer
+chromatography. Quantification of thin layer chromatographs in Figure 4C.
+
+### Supplementary Figure 4 B) source
+https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
+
+<img src=dataset\FigS5A.jpg width=60%>
+
+### Supplementary Figure 5 A) caption
+
+**Supplementary Figure 5 A).** Laminarin from E. bicyclis (2.4 mM) was digested with GBP1 (72 nM) for 1 h at 60 °C.
+The digestion products were analyzed by thin layer chromatography.
+
+### Supplementary Figure 5 A) source
+https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
\ No newline at end of file
-- 
GitLab