diff --git a/assays/EnzymaticCarbohydrateDigestionAndTLC/README.md b/assays/EnzymaticCarbohydrateDigestionAndTLC/README.md index 9a4ee08d0db7e5d20f99dd6fded4e2866150aa17..f4e3b279dbf7f0dbf575ce99b591dc7c765b5a89 100644 --- a/assays/EnzymaticCarbohydrateDigestionAndTLC/README.md +++ b/assays/EnzymaticCarbohydrateDigestionAndTLC/README.md @@ -16,6 +16,25 @@ https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=h ### Figure 4 A) source https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#secsectitle0065 +<img src=dataset\gr4b_lrg.jpg width=60%> + +### Figure 4 B) caption + +**Figure 4 B).** Quantification of GBP1-catalyzed laminarin digestion products. TLC band intensity was quantified using the ImageJ software. + +### Figure 4 B) source +https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#fx1 + +<img src=dataset\gr4c_lrg.jpg width=100%> + +### Figure 4 C) caption + +**Figure 4 C).** Laminarin (4 mg/mL-1) was digested with GBP1 (70 nM) for 10 min at different temperatures (left graph) or in different buffers (10 mM, pH 5–9, right graph). +Products from GBP1-catalyzed laminarin digestion assays were separated by TLC (Figures S4A and S4B) and quantified via ImageJ. UT, untreated. + +### Figure 4 C) source +https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#fx1 + <img src=dataset\FigS4A.jpg width=60%> ### Supplementary Figure 4 A) caption diff --git a/assays/EnzymaticCarbohydrateDigestionAndTLC/dataset/gr4b_lrg.jpg b/assays/EnzymaticCarbohydrateDigestionAndTLC/dataset/gr4b_lrg.jpg new file mode 100644 index 0000000000000000000000000000000000000000..f8bee8cc694350c07dedc1d5bb3fb2feb5f3390f --- /dev/null +++ b/assays/EnzymaticCarbohydrateDigestionAndTLC/dataset/gr4b_lrg.jpg @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:262ff2a38401686cb8c69575cb796a736ce58b89a5f758a55d0baad80d2ed49d +size 39170 diff --git a/assays/EnzymaticCarbohydrateDigestionAndTLC/dataset/gr4c_lrg.jpg b/assays/EnzymaticCarbohydrateDigestionAndTLC/dataset/gr4c_lrg.jpg new file mode 100644 index 0000000000000000000000000000000000000000..20a99c86fb4653023cb8f5921483388d645b648c --- /dev/null +++ b/assays/EnzymaticCarbohydrateDigestionAndTLC/dataset/gr4c_lrg.jpg @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:6b3dd1198a4b75df2b7feec479afa4232ec3a2c0aec517259fa4ea186c8e2126 +size 119804