From 945070bfa5681c49eacad5573fe319e853be80f9 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 20 Jan 2024 13:05:08 +0100
Subject: [PATCH] add figure to TLC assay

---
 .../README.md                                 | 19 +++++++++++++++++++
 .../dataset/gr4b_lrg.jpg                      |  3 +++
 .../dataset/gr4c_lrg.jpg                      |  3 +++
 3 files changed, 25 insertions(+)
 create mode 100644 assays/EnzymaticCarbohydrateDigestionAndTLC/dataset/gr4b_lrg.jpg
 create mode 100644 assays/EnzymaticCarbohydrateDigestionAndTLC/dataset/gr4c_lrg.jpg

diff --git a/assays/EnzymaticCarbohydrateDigestionAndTLC/README.md b/assays/EnzymaticCarbohydrateDigestionAndTLC/README.md
index 9a4ee08..f4e3b27 100644
--- a/assays/EnzymaticCarbohydrateDigestionAndTLC/README.md
+++ b/assays/EnzymaticCarbohydrateDigestionAndTLC/README.md
@@ -16,6 +16,25 @@ https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=h
 ### Figure 4 A) source
 https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#secsectitle0065
 
+<img src=dataset\gr4b_lrg.jpg width=60%>
+
+### Figure 4 B) caption
+
+**Figure 4 B).**  Quantification of GBP1-catalyzed laminarin digestion products. TLC band intensity was quantified using the ImageJ software.
+
+### Figure 4 B) source
+https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#fx1
+
+<img src=dataset\gr4c_lrg.jpg width=100%>
+
+### Figure 4 C) caption
+
+**Figure 4 C).**  Laminarin (4 mg/mL-1) was digested with GBP1 (70 nM) for 10 min at different temperatures (left graph) or in different buffers (10 mM, pH 5–9, right graph).
+Products from GBP1-catalyzed laminarin digestion assays were separated by TLC (Figures S4A and S4B) and quantified via ImageJ. UT, untreated.
+
+### Figure 4 C) source
+https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#fx1
+
 <img src=dataset\FigS4A.jpg width=60%>
 
 ### Supplementary Figure 4 A) caption
diff --git a/assays/EnzymaticCarbohydrateDigestionAndTLC/dataset/gr4b_lrg.jpg b/assays/EnzymaticCarbohydrateDigestionAndTLC/dataset/gr4b_lrg.jpg
new file mode 100644
index 0000000..f8bee8c
--- /dev/null
+++ b/assays/EnzymaticCarbohydrateDigestionAndTLC/dataset/gr4b_lrg.jpg
@@ -0,0 +1,3 @@
+version https://git-lfs.github.com/spec/v1
+oid sha256:262ff2a38401686cb8c69575cb796a736ce58b89a5f758a55d0baad80d2ed49d
+size 39170
diff --git a/assays/EnzymaticCarbohydrateDigestionAndTLC/dataset/gr4c_lrg.jpg b/assays/EnzymaticCarbohydrateDigestionAndTLC/dataset/gr4c_lrg.jpg
new file mode 100644
index 0000000..20a99c8
--- /dev/null
+++ b/assays/EnzymaticCarbohydrateDigestionAndTLC/dataset/gr4c_lrg.jpg
@@ -0,0 +1,3 @@
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+size 119804
-- 
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