diff --git a/README.md b/README.md
index c0e70595e0410253f65474b5617812c03c321f37..f40a5e4f2e3c667e1a1659516fd3a3651a5ab281 100644
--- a/README.md
+++ b/README.md
@@ -19,7 +19,7 @@ Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
   }
 }%%
 
-flowchart TB
+flowchart
 
 subgraph Legend
 l1(Sample)
@@ -136,6 +136,7 @@ d9(gr4a_lrg.jpg)
 end
 
 subgraph assays\QuantificationAndStatisticalAnalysis
+direction TB
 pr25>QuantificationAndStatisticalAnalysis.md]-->d10("`gr6c_lrg.jpg
         mmc3.xlsx`")
 end