diff --git a/assays/CRISPRCas9-basedMutagenesis/isa.assay.xlsx b/assays/CRISPRCas9-basedMutagenesis/isa.assay.xlsx index 67949dc332b98eb845b1f32f02f03ab2e4366fc7..40d7f9c1ce00364c803ed8010955ce8808fc5628 100644 Binary files a/assays/CRISPRCas9-basedMutagenesis/isa.assay.xlsx and b/assays/CRISPRCas9-basedMutagenesis/isa.assay.xlsx differ diff --git a/assays/CRISPRCas9-basedMutagenesis/protocols/CRISPRCas9-basedMutagenesis.md b/assays/CRISPRCas9-basedMutagenesis/protocols/CRISPRCas9-basedMutagenesis.md index 45fd66bcb74d47ca81dc75c08113a638b3d4769d..03c4e47a2758a13374c1f588ead9176f5120d223 100644 --- a/assays/CRISPRCas9-basedMutagenesis/protocols/CRISPRCas9-basedMutagenesis.md +++ b/assays/CRISPRCas9-basedMutagenesis/protocols/CRISPRCas9-basedMutagenesis.md @@ -1,4 +1,5 @@ ## CRISPR/Cas9-based mutagenesis of GBP homologues in barley + The CRISPOR web tool (Concordet et al., 2018) (version 4.97) was used to design two single guide RNAs for each *GBP1* and *GBP2*: GBP1_gRNA1: 5’-CCCGGCACGCTTCTTCGCGCCGG-3’ GBP1_gRNA2: 5’-TGGCGCCTTCGGATGAACAGCGG-3’ diff --git a/assays/EnzymaticCarbohydrateDigestionAndTLC/isa.assay.xlsx b/assays/EnzymaticCarbohydrateDigestionAndTLC/isa.assay.xlsx index b2743589808a50f7852294ca08c34c5b25544815..800cd362c9afabe72f3cf560dcc6ba06b30543c3 100644 Binary files a/assays/EnzymaticCarbohydrateDigestionAndTLC/isa.assay.xlsx and b/assays/EnzymaticCarbohydrateDigestionAndTLC/isa.assay.xlsx differ diff --git a/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.assay.xlsx b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.assay.xlsx index c4b3ee97d23846d16e9d6bf0cd87eaa29487e8a1..cb4f12079cecba442589dce26252ffc36bfdf4d4 100644 Binary files a/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.assay.xlsx and b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.assay.xlsx differ diff --git a/assays/HeterologousProteinProductionAndPurification/isa.assay.xlsx 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diff --git a/assays/MultipleSequenceAlignment/isa.assay.xlsx b/assays/MultipleSequenceAlignment/isa.assay.xlsx index 37332bf09cf751cc83c9840bacea6a07c466205e..e622becd000228dd6de260cc4ed8ff0e87c9a967 100644 Binary files a/assays/MultipleSequenceAlignment/isa.assay.xlsx and b/assays/MultipleSequenceAlignment/isa.assay.xlsx differ diff --git a/assays/OxidativeBurstAssay/isa.assay.xlsx b/assays/OxidativeBurstAssay/isa.assay.xlsx index dffecccd8f092ef9f2627bca42e633944e65bb08..55592a8832ee6c85b2740617bbdafae2a3c93cab 100644 Binary files a/assays/OxidativeBurstAssay/isa.assay.xlsx and b/assays/OxidativeBurstAssay/isa.assay.xlsx differ diff --git a/assays/ProteinPull-down/isa.assay.xlsx b/assays/ProteinPull-down/isa.assay.xlsx index a04e5db0086298abb49e9f1230c0a84abfc0b25a..410789b501aa88b69d9884435398e2e84a3cbbfa 100644 Binary files a/assays/ProteinPull-down/isa.assay.xlsx and b/assays/ProteinPull-down/isa.assay.xlsx differ diff --git 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