From a7a6e9471174f2a9f552de34e04106840182a40a Mon Sep 17 00:00:00 2001 From: Viktoria Petrova <vipet103@hhu.de> Date: Mon, 25 Dec 2023 13:07:18 +0100 Subject: [PATCH] add protocol to study FungalMaterialGrowthConditionsAndBarleyColonizationAssays --- .../protocols/BlumeriaHordei.md | 2 ++ 1 file changed, 2 insertions(+) create mode 100644 studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/BlumeriaHordei.md diff --git a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/BlumeriaHordei.md b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/BlumeriaHordei.md new file mode 100644 index 0000000..0be15e3 --- /dev/null +++ b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/BlumeriaHordei.md @@ -0,0 +1,2 @@ +## Blumeria hordei +*Blumeria hordei* isolate K174 was maintained on intact plants of barley cv. Golden Promise grown in soil at 19 °C, 60% relative humidity and a photoperiod of 14 h with a light intensity of 100 μmol ·m-2 s-1. Control and *GBP* knock-out lines were grown in soil in a growth chamber (poly klima) at 19 °C, 60% relative humidity and a photoperiod of 14 h with a light intensity of 100 μmol ·m−2·s−1. Primary leaves of seven-day-old plants were cut and placed with the adaxial side down on 1% plant agar plates before gravity inoculation with *B. hordei* isolate K1 at a conidial density of about 20 conidia per mm. Leaves were harvested at 48 h and cleared in 70% ethanol before staining of the fungal structures with Coomassie brilliant blue solution (0.1% [w/v] Coomassie brilliant blue R-250 in 50% ethanol and 10% acetic acid) for 10-15 s. Bright field microscopy was used to assess secondary hyphae formation in 50 germinated conidia spores in the tip area and 50 germinated conidia in the middle of the leaf. At least six independent leaves were examined at 48 h after infection for fungal penetration success. \ No newline at end of file -- GitLab