diff --git a/assays/CRISPRCas9-basedMutagenesis/isa.assay.xlsx b/assays/CRISPRCas9-basedMutagenesis/isa.assay.xlsx index 9153f5a92f8ab756844855d8702eeb8826051580..7a3b7f746a98cafbbd74982586c88d590ff7e2c4 100644 Binary files a/assays/CRISPRCas9-basedMutagenesis/isa.assay.xlsx and b/assays/CRISPRCas9-basedMutagenesis/isa.assay.xlsx differ diff --git a/assays/EnzymaticCarbohydrateDigestionAndTLC/isa.assay.xlsx b/assays/EnzymaticCarbohydrateDigestionAndTLC/isa.assay.xlsx index d698551774bd54340001e7b429a159bcfa09647f..0bd8ad2b34520d8d896c7542b536ff7990cded2f 100644 Binary files a/assays/EnzymaticCarbohydrateDigestionAndTLC/isa.assay.xlsx and b/assays/EnzymaticCarbohydrateDigestionAndTLC/isa.assay.xlsx differ diff --git a/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.assay.xlsx b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.assay.xlsx index ec4c79b232b4e5c22410ec62d307f7007b685247..7a443836d02053f0b548d97076e9bd63843bf3d9 100644 Binary files a/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.assay.xlsx and b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.assay.xlsx differ diff --git a/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/RhizophagusIrregularis.md b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/RhizophagusIrregularis.md index 9fe300d6100ddb7c176d601dd3f1e8191aca0f54..91f97af9b5c6585da20d2df1671dc594738b8062 100644 --- a/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/RhizophagusIrregularis.md +++ b/assays/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/protocols/RhizophagusIrregularis.md @@ -1,3 +1,7 @@ ## Rhizophagus irregularis -Barley control and mutant seeds were sterilized and germinated as described previously (Mahdi et al., 2022). Germinated seedlings were transferred to 9 × 9 cm pots containing autoclaved 1:1 silica sand:vermiculite mixture inoculated with 700 mg *R. irregularis* spore inoculum (10,000 spores·g-1 moist diatomaceous earth powder [50% water]) (Symplanta, Darmstadt, Germany). Approximately 500 mg of the inoculum was evenly mixed into the lower two-third substrate layer, further 200 mg were evenly sprinkled into a hole in the upper third of the substrate layer, into which the seedlings were transplanted. The seedlings were grown in the greenhouse and watered weekly with 30 mL of deionized water and tap water in a 1:1 ratio. Roots were harvested at 28 dpi and stored in 50% EtOH at 4 °C until staining. \ No newline at end of file +Barley control and mutant seeds were sterilized and germinated as described previously (Mahdi et al., 2022). Germinated seedlings were transferred to 9 × 9 cm pots containing autoclaved 1:1 silica sand:vermiculite mixture inoculated with 700 mg *R. irregularis* spore inoculum (10,000 spores·g-1 moist diatomaceous earth powder [50% water]) (Symplanta, Darmstadt, Germany). Approximately 500 mg of the inoculum was evenly mixed into the lower two-third substrate layer, further 200 mg were evenly sprinkled into a hole in the upper third of the substrate layer, into which the seedlings were transplanted. The seedlings were grown in the greenhouse and watered weekly with 30 mL of deionized water and tap water in a 1:1 ratio. Roots were harvested at 28 dpi and stored in 50% EtOH at 4 °C until staining. + +## Staining + +Roots were stained according to a previously published protocol (Vierheilig et al., 1998). Briefly, roots were incubated for 15 min at 95 °C in 10% KOH, washed with 10% acetic acid and incubated for 5 min at 95 °C with a staining solution of 5% ink (Pelikan, Falkensee, Germany) in 5% acetic acid. After staining, the roots were carefully washed with tap water, then incubated in 5% acetic acid at 4 °C for at least 20 min. The ink-stained root tissue was cut into 1 cm segments with a scalpel and 30 segments of similar diameter were randomly selected from each genotype. Cross-section points were determined from 10 random cuts per root segment. Ink-stained *R. irregularis* structures such as intraradical hyphae (IRH), extraradical hyphae (ERH), arbuscules and vesicles were visualized with a light microscope (AxioStar, Carl Zeiss, Jena, Germany) at 10× magnification. Colonization with R. irregularis was scored as positive if IRH, arbuscules or vesicles were present. The roots of four biological replicates per genotype were examined. \ No newline at end of file diff --git a/assays/HeterologousProteinProductionAndPurification/isa.assay.xlsx b/assays/HeterologousProteinProductionAndPurification/isa.assay.xlsx index d4f2acd5b7134daeccdf7eedd29546d43fd931e8..022bcc5ca05821d5e1c2922669fe051ffda09023 100644 Binary files a/assays/HeterologousProteinProductionAndPurification/isa.assay.xlsx and b/assays/HeterologousProteinProductionAndPurification/isa.assay.xlsx differ diff --git a/assays/MALDI-TOFAnalysis/isa.assay.xlsx b/assays/MALDI-TOFAnalysis/isa.assay.xlsx index 90164aee9c065f3b0a8bf25dca693bbd78569ded..7cf3179a09c3a1a917793b00b3d3c8d544b76c46 100644 Binary files a/assays/MALDI-TOFAnalysis/isa.assay.xlsx and b/assays/MALDI-TOFAnalysis/isa.assay.xlsx differ diff --git a/assays/MS-MSAnalysisOfThePull-downProteins/isa.assay.xlsx b/assays/MS-MSAnalysisOfThePull-downProteins/isa.assay.xlsx index ef28671582092aafeb5e5f24eb1843c86353bcfd..c4443ac630a4ed81cde5e0eb4bf4ac0e394f5b53 100644 Binary files a/assays/MS-MSAnalysisOfThePull-downProteins/isa.assay.xlsx and b/assays/MS-MSAnalysisOfThePull-downProteins/isa.assay.xlsx differ diff --git a/assays/MultipleSequenceAlignment/isa.assay.xlsx b/assays/MultipleSequenceAlignment/isa.assay.xlsx index da568d71ca17a0dee021b359af00504c63d68d5a..b29fe0d202042362709b93911f2e6d87411b0bf3 100644 Binary files a/assays/MultipleSequenceAlignment/isa.assay.xlsx and b/assays/MultipleSequenceAlignment/isa.assay.xlsx differ diff --git a/assays/OxidativeBurstAssay/isa.assay.xlsx b/assays/OxidativeBurstAssay/isa.assay.xlsx index 470d26a4bee877075d03163722d7bb6844dff2a3..72ad3492526a05f4682b403eacb8a9215aaa79b3 100644 Binary files a/assays/OxidativeBurstAssay/isa.assay.xlsx and b/assays/OxidativeBurstAssay/isa.assay.xlsx differ diff --git a/assays/PlasmidConstructionForHeterologousProteinProduction/isa.assay.xlsx b/assays/PlasmidConstructionForHeterologousProteinProduction/isa.assay.xlsx index 8b20dbb6ab0d65bba9438ae49af25d6b11d4eff8..81be3595f0b16aa65e8936e234952ac2af40f7b1 100644 Binary files a/assays/PlasmidConstructionForHeterologousProteinProduction/isa.assay.xlsx and b/assays/PlasmidConstructionForHeterologousProteinProduction/isa.assay.xlsx differ diff --git a/assays/ProteinPull-down/isa.assay.xlsx b/assays/ProteinPull-down/isa.assay.xlsx index 2d565de629c9d37ca003f8f076557e37275e4abe..7920bae837af53796ce4a0fe11419fa1c36ccb35 100644 Binary files a/assays/ProteinPull-down/isa.assay.xlsx and b/assays/ProteinPull-down/isa.assay.xlsx differ diff --git a/assays/QuantificationAndStatisticalAnalyses/isa.assay.xlsx b/assays/QuantificationAndStatisticalAnalyses/isa.assay.xlsx index 424f4c8e63ce48dad058c3a96f1eb74f4070e84e..9616b1ec4c0117b82efe19590fa70ccc3e39b0a4 100644 Binary files a/assays/QuantificationAndStatisticalAnalyses/isa.assay.xlsx and b/assays/QuantificationAndStatisticalAnalyses/isa.assay.xlsx differ diff --git a/assays/StainingForConfocalMicroscopy/isa.assay.xlsx b/assays/StainingForConfocalMicroscopy/isa.assay.xlsx index ee3e4f5a464d537861036697bda0607b247cabe9..d06163cc85da3818d0a1ca98cbde8ecbbf9f90d1 100644 Binary files a/assays/StainingForConfocalMicroscopy/isa.assay.xlsx and b/assays/StainingForConfocalMicroscopy/isa.assay.xlsx differ diff --git a/assays/StainingForConfocalMicroscopy/protocols/RootStainingOfRIrregularis.md b/assays/StainingForConfocalMicroscopy/protocols/RootStainingOfRIrregularis.md deleted file mode 100644 index a3b138a97d4297b788be4871e7aac218dd264746..0000000000000000000000000000000000000000 --- a/assays/StainingForConfocalMicroscopy/protocols/RootStainingOfRIrregularis.md +++ /dev/null @@ -1,3 +0,0 @@ -## Root staining of *R. irregularis* - -Roots were stained according to a previously published protocol (Vierheilig et al., 1998). Briefly, roots were incubated for 15 min at 95 °C in 10% KOH, washed with 10% acetic acid and incubated for 5 min at 95 °C with a staining solution of 5% ink (Pelikan, Falkensee, Germany) in 5% acetic acid. After staining, the roots were carefully washed with tap water, then incubated in 5% acetic acid at 4 °C for at least 20 min. The ink-stained root tissue was cut into 1 cm segments with a scalpel and 30 segments of similar diameter were randomly selected from each genotype. Cross-section points were determined from 10 random cuts per root segment. Ink-stained *R. irregularis* structures such as intraradical hyphae (IRH), extraradical hyphae (ERH), arbuscules and vesicles were visualized with a light microscope (AxioStar, Carl Zeiss, Jena, Germany) at 10× magnification. Colonization with R. irregularis was scored as positive if IRH, arbuscules or vesicles were present. The roots of four biological replicates per genotype were examined. \ No newline at end of file diff --git a/assays/StainingForConfocalMicroscopy/protocols/HordeumVulgare.md b/assays/StainingForConfocalMicroscopy/protocols/StainingProtocolForConfocalMicroscopy.md similarity index 100% rename from assays/StainingForConfocalMicroscopy/protocols/HordeumVulgare.md rename to assays/StainingForConfocalMicroscopy/protocols/StainingProtocolForConfocalMicroscopy.md diff --git a/assays/qRT-PCR/isa.assay.xlsx b/assays/qRT-PCR/isa.assay.xlsx index bbeea963133fd97b5eb2d5e23bd6cf354abfb1c5..c05a1edd46f713d034efdbb02409700ffb6e1200 100644 Binary files a/assays/qRT-PCR/isa.assay.xlsx and b/assays/qRT-PCR/isa.assay.xlsx differ diff --git a/isa.investigation.xlsx b/isa.investigation.xlsx index d0771cfb55ff2857afe33c39a8e92dc97e01ef4f..238b7c416d276adf3c6d0c671bbb63220be376ee 100644 Binary files a/isa.investigation.xlsx and b/isa.investigation.xlsx differ diff --git a/studies/CarbohydrateSubstratesForImmunityAndEnzymaticDigestionAssays/isa.study.xlsx b/studies/CarbohydrateSubstratesForImmunityAndEnzymaticDigestionAssays/isa.study.xlsx index 6f0f9c988ecac74201e530c1d97362c4953d76c7..687f244ac65b3c6d34b509a2b95200b7dd48a24e 100644 Binary files a/studies/CarbohydrateSubstratesForImmunityAndEnzymaticDigestionAssays/isa.study.xlsx and b/studies/CarbohydrateSubstratesForImmunityAndEnzymaticDigestionAssays/isa.study.xlsx differ diff --git a/studies/HordeumVulgare/isa.study.xlsx b/studies/HordeumVulgare/isa.study.xlsx index 2bd292a79a688cf4098001964cd054ddf801a01c..f8fa880394ab3230460ff2cae93bc930404fc784 100644 Binary files a/studies/HordeumVulgare/isa.study.xlsx and b/studies/HordeumVulgare/isa.study.xlsx differ diff --git a/studies/NicotianaBenthamiana/isa.study.xlsx b/studies/NicotianaBenthamiana/isa.study.xlsx index f1c46e874ca63887937cd616fe847f50645126b7..ed58ec5782d63a9a108c97646eea5f89af0bcb4c 100644 Binary files a/studies/NicotianaBenthamiana/isa.study.xlsx and b/studies/NicotianaBenthamiana/isa.study.xlsx differ