diff --git a/assays/MS-MSAnalysisOfThePull-downProteins/isa.assay.xlsx b/assays/MS-MSAnalysisOfThePull-downProteins/isa.assay.xlsx index dc96192068ba10f80e7edc7189463a624b523e01..5da3929640b717f566d608a88f5707a5e1de42af 100644 Binary files a/assays/MS-MSAnalysisOfThePull-downProteins/isa.assay.xlsx and b/assays/MS-MSAnalysisOfThePull-downProteins/isa.assay.xlsx differ diff --git a/assays/OxidativeBurstAssay/README.md b/assays/OxidativeBurstAssay/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/OxidativeBurstAssay/dataset/.gitkeep b/assays/OxidativeBurstAssay/dataset/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/OxidativeBurstAssay/isa.assay.xlsx b/assays/OxidativeBurstAssay/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..079eac191b001fc238d086b7f8a5b0bdfb056aa3 Binary files /dev/null and b/assays/OxidativeBurstAssay/isa.assay.xlsx differ diff --git a/assays/OxidativeBurstAssay/protocols/.gitkeep b/assays/OxidativeBurstAssay/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/OxidativeBurstAssay/protocols/ROSBurstAssay.md b/assays/OxidativeBurstAssay/protocols/ROSBurstAssay.md new file mode 100644 index 0000000000000000000000000000000000000000..57a3a5ac0a78a1df452202fbe5d65dd682bedbc3 --- /dev/null +++ b/assays/OxidativeBurstAssay/protocols/ROSBurstAssay.md @@ -0,0 +1,2 @@ +## ROS burst assay +The ROS burst assay was based on previously published protocols.33,85 In brief, a 96-well plate containing water and plant material (as described above) was incubated overnight at RT to remove ROS that had resulted from mechanical damage to the tissue during preparation. The next day, the water was replaced with 100 μL of fresh Milli-Q water containing 20 μg·mL-1 horseradish peroxidase (Sigma-Aldrich, Taufkirchen, Germany) and 20 μM L-012 (Wako Chemicals, Neuss, Germany). After a short incubation period (∼15 min), 100 μL of double-concentrated elicitor solutions were added to the wells. All elicitors were dissolved in Milli-Q water without additional treatment. Measurements of elicitor-triggered apoplastic ROS production were started immediately and performed continuously with an integration time of 450 ms in a TECAN SPARK 10 M multiwell plate reader (Männedorf, Switzerland). diff --git a/isa.investigation.xlsx b/isa.investigation.xlsx index 9436c8d2c4f39fc20689a729dd1ec05306f30dcf..1c5b25406e0b0196214bd30d95f23270918796e0 100644 Binary files a/isa.investigation.xlsx and b/isa.investigation.xlsx differ diff --git a/studies/Barley/isa.study.xlsx b/studies/Barley/isa.study.xlsx index 6619ed5420c0bf8aca2616c78a5b9a170657ff9a..dfe27555ffe70166472148c968e6ae60385cfc29 100644 Binary files a/studies/Barley/isa.study.xlsx and b/studies/Barley/isa.study.xlsx differ diff --git a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.study.xlsx b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.study.xlsx index a3f8ea6af84512ec6d848021f668464e915a717d..9746ba5b1312c8b45bb946138b741920ac29cf1e 100644 Binary files a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.study.xlsx and b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.study.xlsx differ diff --git a/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx b/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx index 29278d6f3abf72f0ef0057da7f2d9191da9d3eb0..5e88e58d545a8b38164e29ebc0987f28c78db3ef 100644 Binary files a/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx and b/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx differ