diff --git a/isa.investigation.xlsx b/isa.investigation.xlsx index c411a7bdeac6bcf80cdbc2e9e7b5dd9781d94fe1..41f7f0519ad12e89e7faf90cb61d168654928f7f 100644 Binary files a/isa.investigation.xlsx and b/isa.investigation.xlsx differ diff --git a/studies/Barley/isa.study.xlsx b/studies/Barley/isa.study.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..79933d3a2d37bbd1988dbef967898a74c007bb50 Binary files /dev/null and b/studies/Barley/isa.study.xlsx differ diff --git a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.study.xlsx b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.study.xlsx index 76223bfdc088280afd03c8cd1cd9e752be055152..d252a07f4395555e72e5409b4037de3e85aadb62 100644 Binary files a/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.study.xlsx and b/studies/FungalMaterialGrowthConditionsAndBarleyColonizationAssays/isa.study.xlsx differ diff --git a/studies/HordeumVulgare/protocols/PlantMaterialAndGrowthConditions.md b/studies/HordeumVulgare/protocols/PlantMaterialAndGrowthConditions.md new file mode 100644 index 0000000000000000000000000000000000000000..35fec13a34c70f049b7ad0874b79880293b9ff55 --- /dev/null +++ b/studies/HordeumVulgare/protocols/PlantMaterialAndGrowthConditions.md @@ -0,0 +1,2 @@ +## PlantMaterialAndGrowthConditions +All experiments, including the generation of CRISPR/Cas9 knock-out lines, were performed with the spring barley (*H. vulgare L.*) cv. Golden Promise Fast, an introgression line carrying the Ppd-H1 allele that confers fast flowering (Gol et al., 2021) From here on, we use “control†to name this non-mutagenized cultivar that carries normal copies of *GBP1* and *GBP2*. For ROS burst assays, barley seeds were surface sterilized with 6% sodium hypochlorite for 1 h and then washed extensively (5 × 30 mL sterile water). Seeds were germinated on wet filter paper at room temperature in the dark under sterile conditions for three days before transfer to sterile jars containing solid 1/10 plant nutrition medium (PNM), pH 5.7 and 0.4% Gelrite (Duchefa, Haarlem, the Netherlands).82 Seedlings were cultured for four days in a growth chamber under long-day conditions (day/night cycle of 16/8 h, 22 °C/18 °C, light intensity of 108 μmol·m−2·s−1). \ No newline at end of file diff --git a/studies/NicotianaBenthamiana/isa.study.xlsx b/studies/NicotianaBenthamiana/isa.study.xlsx index 238c0cf55e2d0febbec6e9f7bb2cfafb0ad63c31..605be735b085adfb4e212e6bf2f3a42113b6ec9e 100644 Binary files a/studies/NicotianaBenthamiana/isa.study.xlsx and b/studies/NicotianaBenthamiana/isa.study.xlsx differ diff --git a/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx b/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx index 75900910e6d429bbfe4b544cebaf22878305fd92..db6f51b41a4bf958a728a5834c424761406c2601 100644 Binary files a/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx and b/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx differ