diff --git a/isa.investigation.xlsx b/isa.investigation.xlsx index 41f7f0519ad12e89e7faf90cb61d168654928f7f..dead9f81829e6cbb5bfe7bdd9fd296139184cb0a 100644 Binary files a/isa.investigation.xlsx and b/isa.investigation.xlsx differ diff --git a/studies/Barley/isa.study.xlsx b/studies/Barley/isa.study.xlsx deleted file mode 100644 index 79933d3a2d37bbd1988dbef967898a74c007bb50..0000000000000000000000000000000000000000 Binary files a/studies/Barley/isa.study.xlsx and /dev/null differ diff --git a/studies/PlantMaterialAndGrowthConditions/README.md b/studies/PlantMaterialAndGrowthConditions/README.md deleted file mode 100644 index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..0000000000000000000000000000000000000000 diff --git a/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx b/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx deleted file mode 100644 index db6f51b41a4bf958a728a5834c424761406c2601..0000000000000000000000000000000000000000 Binary files a/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx and /dev/null differ diff --git a/studies/PlantMaterialAndGrowthConditions/protocols/.gitkeep b/studies/PlantMaterialAndGrowthConditions/protocols/.gitkeep deleted file mode 100644 index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..0000000000000000000000000000000000000000 diff --git a/studies/PlantMaterialAndGrowthConditions/protocols/HordeumVulgare.md b/studies/PlantMaterialAndGrowthConditions/protocols/HordeumVulgare.md deleted file mode 100644 index 70b001d305bf1ed4a555192e35ff1c477402e46b..0000000000000000000000000000000000000000 --- a/studies/PlantMaterialAndGrowthConditions/protocols/HordeumVulgare.md +++ /dev/null @@ -1,2 +0,0 @@ -## Hordeum vulgare -All experiments, including the generation of CRISPR/Cas9 knock-out lines, were performed with the spring barley (*H. vulgare L.*) cv. Golden Promise Fast, an introgression line carrying the Ppd-H1 allele that confers fast flowering.42 From here on, we use “control†to name this non-mutagenized cultivar that carries normal copies of *GBP1* and *GBP2*. For ROS burst assays, barley seeds were surface sterilized with 6% sodium hypochlorite for 1 h and then washed extensively (5 × 30 mL sterile water). Seeds were germinated on wet filter paper at room temperature in the dark under sterile conditions for three days before transfer to sterile jars containing solid 1/10 plant nutrition medium (PNM), pH 5.7 and 0.4% Gelrite (Duchefa, Haarlem, the Netherlands).82 Seedlings were cultured for four days in a growth chamber under long-day conditions (day/night cycle of 16/8 h, 22 °C/18 °C, light intensity of 108 μmol·m−2·s−1). \ No newline at end of file diff --git a/studies/PlantMaterialAndGrowthConditions/protocols/NicotianaBenthamiana.md b/studies/PlantMaterialAndGrowthConditions/protocols/NicotianaBenthamiana.md deleted file mode 100644 index 49317fc2104e4d9df6b7f589eb47e2b222a95350..0000000000000000000000000000000000000000 --- a/studies/PlantMaterialAndGrowthConditions/protocols/NicotianaBenthamiana.md +++ /dev/null @@ -1,2 +0,0 @@ -## Nicotiana benthamiana -Seeds of *N. benthamiana* wild-type lines were sown on soil and grown for 3 weeks in the greenhouse under long-day conditions (day/night cycle of 16/8 h, 22–25 °C, light intensity of ∼140 μmol·m−2·s−1, maximal humidity of 60%). \ No newline at end of file diff --git a/studies/PlantMaterialAndGrowthConditions/resources/.gitkeep b/studies/PlantMaterialAndGrowthConditions/resources/.gitkeep deleted file mode 100644 index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..0000000000000000000000000000000000000000