A GH81-type β-glucan-binding protein enhances colonization by mutualistic fungi in barley
Original publication
Wanke, A., van Boerdonk, S., Mahdi, L. K., Wawra, S., Neidert, M., Chandrasekar, B., Saake, P., Saur, I. M. L., Derbyshire, P., Holton, N., Menke, F. L. H., Brands, M., Pauly, M., Acosta, I. F., Zipfel, C., & Zuccaro, A. (2023). A GH81-type β-glucan-binding protein enhances colonization by mutualistic fungi in barley. Current biology : CB, 33(23), 5071–5084.e7. https://doi.org/10.1016/j.cub.2023.10.048
License
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Data availability
Lead Contact
Further information and requests for resources, reagents, datasets, and protocols should be directed to and will be fulfilled by the lead contact, Alga Zuccaro (azuccaro@uni-koeln.de).
Materials Availability
Plasmids and barley lines generated in this study are available upon request and will be provided by the lead contact, Alga Zuccaro (azuccaro@uni-koeln.de).
Data and Code Availability
Proteomic data has been deposited at the ProteomeXchange Consortium via PRIDE partner repository (Perez-Riverol et al., 2019) and are publicly available as of the date of publication. Accession numbers are listed in the key resources table.
This paper does not report original code.
Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.
ARC structure
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flowchart
subgraph Legend
l1(Sample)
l2>Process]
l3(Material)
l4(Data)
end
subgraph studies\NicotianaBenthamiana
direction TB
s1(wild type seeds)---p1>PlantMaterialAndGrowthConditions.md]-->s2(N. benthamiana plants)
end
subgraph studies\HordeumVulgare
direction TB
s3(barley seeds)---p2>PlantMaterialAndGrowthConditions.md]-->s4(barley plants)
end
subgraph studies\CarbohydrateSubstratesForImmunityAndEnzymaticDigestionAssays
direction TB
p3>CarbohydrateSubstratesForImmunityAndEnzymaticDigestionAssays.md]-->s5(carbohydrate substrates)
end
subgraph assays\HeterologousProteinProductionAndPurification
direction TB
a1(codon-optimized GBP1 sequence)---p4>PlasmidConstruction.md]-->a2(pXCScpmv-GBP1-HAStrep)
a3(catalitically inactive GBP1 sequence)---p4-->a4(pXCScpmv-GBP1_E500A-HAStrep)
a2----p6>"`isolation
sequencing
_Agrobacterium tumefaciens_ transformation`"]
a4---p5>"`gel electrophoresis
purification
_E.coli_ transformation`"]---p6
p6-->a5(Agrobacterium_GBP1)
p6-->a6(Agrobacterium_GBP1_E500A)
a5 & a6---p7>infiltration]
s2----p7
p7---p8>HeterologousProteinProductionAndPurification.md]-->a7(purified proteins)
end
subgraph assays\EnzymaticCarbohydrateDigestionAndTLC
direction TB
a7 & s5---p9>EnzymaticCarbohydrateDigestion.md]-->a8(digestion products)
a8---p10>TLC.md]-->d1("`gr3b_lrg.jpg
gr4a_lrg.jpg
gr4b_lrg.jpg
gr4c_lrg.jpg
FigS4A.jpg
FigS4B.jpg
FigS5A.png`")
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subgraph assays\MALDI-TOFAnalysis
direction TB
a8---p11>MALDI-TOFAnalysis.md]-->d2("`gr3c_large.jpg
FigS4C.jpg`")
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subgraph assays\CRISPRCas9-basedMutagenesis
direction TB
s4---p12>CRISPRCas9-basedMutagenesis.md]-->a9(barley mutants)-->d15(gr1b_lrg.jpg)
end
subgraph assays\OxidativeBurstAssay
direction TB
s2 & s4 & a9---p13>PreparationOfThePlantMaterial.md]-->a10(plant material)
a10---p14>ROSBurstAssay.md]-->d3("`gr5_lrg.jpg
FigS1A.jpg
FigS1B.jpg
FigS5B.jpg
FigS5C.jpg`")
end
subgraph assays\FungalMaterialGrowthConditionsAndBarleyColonizationAssays
direction TB
s4 & a9---p15>RhizophagusIrregularis.md]-->a11(R. irregularis colonized plants)-->d11(FigS3B.jpg)
s4 & a9---p16>BlumeriaHordei.md]-->a12(B. hordei colonized plants)-->d13(FigS3D.jpg)
s4 & a9---p17>SerendipitaIndicaSerendipitaVermiferaAndBipolarisSorokiniana.md]-->a13(S. indica colonized plants) & a14(S. vermifera colonized plants) & a15(B. sorokiniana colonized plants)
a15-->d12(FigS3C.jpg)
d14(gr6b_lrg.jpg)
end
subgraph assays\qRT-PCR
direction TB
a12 & a13 & a14 & a15---p18>qRT-PCR.md]-->d4("`gr1c_lrg.jpg
gr2_lrg.jpg
gr6d_lrg.jpg`")
end
subgraph assays\StainingForConfocalMicroscopy
direction TB
a13---p19>HordeumVulgare.md]-->d5("`gr6a_lrg.jpg
FigS6A.jpg
FigS6B.jpg
FigS6C.jpg`")
a11---p20>RootStainingOfRIrregularis.md]-->d6(FigS3A.jpg)
end
subgraph assays\ProteinPull-down
direction TB
a16(laminarin)---p21>BiotinylationOfLaminarin.md]-->a17(biotinylated laminarin)
s4 & a17---p22>ProteinPull-downWithBiotinylatedLaminarin.md]-->a18(pull-down proteins)
a18---p23>SDS-PAGE separation]-->d7(FigS1C.jpg)
end
subgraph assays\MS-MSAnalysisOfThePull-downProteins
direction TB
a18---p24>MS-MSAnalysisOfThePull-downProteins.md]-->d8("`gr1a_lrg.jpg
mmc2.xlsx`")
end
subgraph assays\MultipleSequenceAlignment
d9(gr4a_lrg.jpg)
end
subgraph assays\QuantificationAndStatisticalAnalysis
direction TB
pr25>QuantificationAndStatisticalAnalysis.md]-->d10("`gr6c_lrg.jpg
mmc3.xlsx`")
end
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