A GH81-type β-glucan-binding protein enhances colonization by mutualistic fungi in barley
Original publication
Wanke, A., van Boerdonk, S., Mahdi, L. K., Wawra, S., Neidert, M., Chandrasekar, B., Saake, P., Saur, I. M. L., Derbyshire, P., Holton, N., Menke, F. L. H., Brands, M., Pauly, M., Acosta, I. F., Zipfel, C., & Zuccaro, A. (2023). A GH81-type β-glucan-binding protein enhances colonization by mutualistic fungi in barley. Current biology : CB, 33(23), 5071–5084.e7. https://doi.org/10.1016/j.cub.2023.10.048
License
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Data availability
Lead Contact
Further information and requests for resources, reagents, datasets, and protocols should be directed to and will be fulfilled by the lead contact, Alga Zuccaro (azuccaro@uni-koeln.de).
Materials Availability
Plasmids and barley lines generated in this study are available upon request and will be provided by the lead contact, Alga Zuccaro (azuccaro@uni-koeln.de).
Data and Code Availability
Proteomic data has been deposited at the ProteomeXchange Consortium via PRIDE partner repository (Perez-Riverol et al., 2019) and are publicly available as of the date of publication. Accession numbers are listed in the key resources table.
This paper does not report original code.
Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.
ARC structure
flowchart
subgraph CarbohydrateSubstratesForImmunityAndEnzymaticDigestionAssays
end
subgraph HordeumVulgare
ROS_burst_assay_preparation
germination
growth_chamber
end
subgraph NicotianaBenthamiana
greenhouse_conditions
end
subgraph HeterologousProteinProductionAndPurification
Agrobacterium_growth
Nicotiana_infiltration
ground_material
incubation
purification
end
subgraph PlasmidConstructionForHeterologousProteinProduction
amplification
cloning
site-directed_mutagenesis_PCR
purification_and_E.coli_transfo
Agrobacterium_transformation
end
subgraph ProteinPull-down
biothinylation_of_laminarin
extraction
incubation
pull-down
SDS-PAGE
end
subgraph OxidativeBurstAssay
preparation_of_plant_material
ROS_burst_assay
end
subgraph MALDI-TOFAnalysis
MALDI-TOF
end
subgraph EnzymaticCarbohydrateDigestionAndTLC
EnzymaticCarbohydrateDigestion
TLC
end
subgraph StainingForConfocalMicroscopy
staining
end
subgraph CRISPRCas9-basedMutagenesis
expression_cassettes
plasmid_generation
CRISPR_Cas9_mutagenesis
end
subgraph MultipleSequenceAlignment
alignment
end
subgraph MS-MSAnalysisOfThePull-downProteins
MS-MS_analysis
end
subgraph FungalMaterialGrowthConditionsAndBarleyColonizationAssays
R.irregularis
R.irregularis_staining
Si_Sv_Bs
B.hordei
B.hordei_staining
end
subgraph QuantificationAndStatisticalAnalyses
end
subgraph qRT-PCR
RT-PCR
end
Wanke-2023 --> CarbohydrateSubstratesForImmunityAndEnzymaticDigestionAssays
Wanke-2023 --> HordeumVulgare
Wanke-2023 --> NicotianaBenthamiana
ROS_burst_assay_preparation --3--> germination
germination --3--> growth_chamber
growth_chamber --3--> preparation_of_plant_material
growth_chamber --1--> R.irregularis
growth_chamber --1--> Si_Sv_Bs
growth_chamber --1--> B.hordei
greenhouse_conditions --1--> preparation_of_plant_material
Agrobacterium_growth --3--> Nicotiana_infiltration
Nicotiana_infiltration --1--> ground_material
ground_material --1--> incubation
incubation --1--> purification
purification --2--> EnzymaticCarbohydrateDigestion
amplification --1--> cloning
cloning --1--> site-directed_mutagenesis_PCR
cloning --1--> Agrobacterium_transformation
site-directed_mutagenesis_PCR --1--> purification_and_E.coli_transfo
purification_and_E.coli_transfo --1--> Agrobacterium_transformation
Agrobacterium_transformation --2--> Agrobacterium_growth
biothinylation_of_laminarin --1--> extraction
extraction --1--> incubation
incubation --6--> pull-down
pull-down --6--> SDS-PAGE
SDS-PAGE --5--> MS-MS_analysis
preparation_of_plant_material --6--> ROS_burst_assay
EnzymaticCarbohydrateDigestion --28--> MALDI-TOF
EnzymaticCarbohydrateDigestion --28--> TLC
expression_cassettes --3--> plasmid_generation
plasmid_generation --1--> CRISPR_Cas9_mutagenesis
CRISPR_Cas9_mutagenesis --2--> ROS_burst_assay_preparation
R.irregularis --3--> R.irregularis_staining
Si_Sv_Bs --3--> staining
Si_Sv_Bs --9--> RT-PCR
B.hordei --3--> B.hordei_staining