diff --git a/assays/CollectionOfRootExudates/protocols/CollectionOfRootExudatesProtocol.md b/assays/CollectionOfRootExudates/protocols/CollectionOfRootExudatesProtocol.md index c60de24f124da7fc243d381dbcee2934a82a5cbe..fa8c68c014811567e2bd6451c339854a6d43f942 100644 --- a/assays/CollectionOfRootExudates/protocols/CollectionOfRootExudatesProtocol.md +++ b/assays/CollectionOfRootExudates/protocols/CollectionOfRootExudatesProtocol.md @@ -1,3 +1,3 @@ ## Collection of root exudates -*Arabidopsis* and *Lotus* plants were grown in a customized hydroponic system (original design by M. Peukert, University of Cologne, unpublished). This sterile growth setup consists of glass jars filled with glass beads and a stainless-steel mesh on top. Nutrient solution (modified 0.25× B&D medium, Fe-EDTA instead of Fe-citrate) was poured into the jars until the beads were covered in liquid and the liquid touched the metal mesh. We used the same medium for both plant species to allow for direct comparison of exudate composition, and to minimize differential effects on the bacterial community originating from different media types. We chose the *Lotus* B&D medium since *Arabidopsis* grew reasonably well in it. Sterilized and pregerminated seeds were placed onto the mesh, jars were put into sterile plastic boxes with filter lids (SacO2 microboxes) and plants were grown for 5 weeks. The medium containing root exudates was removed from the jars in the clean bench using a sterile metal needle and plastic syringe. After transfer to 50-ml Falcon tubes, exudates were frozen at −80 °C, freeze-dried until a volume of 2–3 ml was left, thawed and adjusted with sterile water to 5 ml. Exudates were kept at −80 °C until further usage. \ No newline at end of file +*Arabidopsis* and *Lotus* plants were grown in a customized hydroponic system (original design by M. Peukert, University of Cologne, unpublished). This sterile growth setup consists of glass jars filled with glass beads and a stainless-steel mesh on top. Nutrient solution (modified 0.25× B&D medium, Fe-EDTA instead of Fe-citrate) was poured into the jars until the beads were covered in liquid and the liquid touched the metal mesh. We used the same medium for both plant species to allow for direct comparison of exudate composition, and to minimize differential effects on the bacterial community originating from different media types. We chose the *Lotus* B&D medium since *Arabidopsis* grew reasonably well in it. Sterilized and pregerminated seeds were placed onto the mesh, jars were put into sterile plastic boxes with filter lids (SacO2 microboxes) and plants were grown for 5 weeks. The medium containing root exudates was removed from the jars in the clean bench using a sterile metal needle and plastic syringe. After transfer to 50-ml Falcon tubes, exudates were frozen at −80 °C, freeze-dried until a volume of 2–3 ml was left, thawed and adjusted with sterile water to 5 ml. Exudates were kept at −80 °C until further usage. \ No newline at end of file diff --git a/assays/DeadRootExperiment/isa.assay.xlsx b/assays/DeadRootExperiment/isa.assay.xlsx index 0f4831c25314e15270e1013810924b85bcc2ab6f..2563c9656c2cc9238814092c58056358d86ea41a 100644 Binary files a/assays/DeadRootExperiment/isa.assay.xlsx and b/assays/DeadRootExperiment/isa.assay.xlsx differ diff --git a/assays/GreenhouseExperiment/isa.assay.xlsx b/assays/GreenhouseExperiment/isa.assay.xlsx index ce191913f0b646157de87ac2d0afa66f09496f14..7213cbd14da431c2f5fed52b521760032268835f 100644 Binary files a/assays/GreenhouseExperiment/isa.assay.xlsx and b/assays/GreenhouseExperiment/isa.assay.xlsx differ diff --git a/studies/BacterialAndPlantMaterial/isa.study.xlsx b/studies/BacterialAndPlantMaterial/isa.study.xlsx index 1cca99b5abcac7c23d8ad59ce6819678998db9b8..6b11dd08020be82f03a51a5776b49ab015ee164d 100644 Binary files a/studies/BacterialAndPlantMaterial/isa.study.xlsx and b/studies/BacterialAndPlantMaterial/isa.study.xlsx differ diff --git a/studies/LotusJaponicusCultureCollection/isa.study.xlsx b/studies/LotusJaponicusCultureCollection/isa.study.xlsx index 599fddd4351d959517d45d954cdb863159ee194c..6fb0d6dfe5c6b0a91f9620d5e6a692c6ddc2961e 100644 Binary files a/studies/LotusJaponicusCultureCollection/isa.study.xlsx and b/studies/LotusJaponicusCultureCollection/isa.study.xlsx differ