diff --git a/assays/CollectionOfRootExudates/README.md b/assays/CollectionOfRootExudates/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/CollectionOfRootExudates/dataset/.gitkeep b/assays/CollectionOfRootExudates/dataset/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/CollectionOfRootExudates/isa.assay.xlsx b/assays/CollectionOfRootExudates/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..087b001321c85e9b27214d39e487155609f9d74c Binary files /dev/null and b/assays/CollectionOfRootExudates/isa.assay.xlsx differ diff --git a/assays/CollectionOfRootExudates/protocols/.gitkeep b/assays/CollectionOfRootExudates/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/CollectionOfRootExudates/protocols/CollectionOfRootExudatesProtocol.md b/assays/CollectionOfRootExudates/protocols/CollectionOfRootExudatesProtocol.md new file mode 100644 index 0000000000000000000000000000000000000000..c60de24f124da7fc243d381dbcee2934a82a5cbe --- /dev/null +++ b/assays/CollectionOfRootExudates/protocols/CollectionOfRootExudatesProtocol.md @@ -0,0 +1,3 @@ +## Collection of root exudates + +*Arabidopsis* and *Lotus* plants were grown in a customized hydroponic system (original design by M. Peukert, University of Cologne, unpublished). This sterile growth setup consists of glass jars filled with glass beads and a stainless-steel mesh on top. Nutrient solution (modified 0.25× B&D medium, Fe-EDTA instead of Fe-citrate) was poured into the jars until the beads were covered in liquid and the liquid touched the metal mesh. We used the same medium for both plant species to allow for direct comparison of exudate composition, and to minimize differential effects on the bacterial community originating from different media types. We chose the *Lotus* B&D medium since *Arabidopsis* grew reasonably well in it. Sterilized and pregerminated seeds were placed onto the mesh, jars were put into sterile plastic boxes with filter lids (SacO2 microboxes) and plants were grown for 5 weeks. The medium containing root exudates was removed from the jars in the clean bench using a sterile metal needle and plastic syringe. After transfer to 50-ml Falcon tubes, exudates were frozen at −80 °C, freeze-dried until a volume of 2–3 ml was left, thawed and adjusted with sterile water to 5 ml. Exudates were kept at −80 °C until further usage. \ No newline at end of file diff --git a/assays/SynComInvasionExperiments/isa.assay.xlsx b/assays/SynComInvasionExperiments/isa.assay.xlsx index 72c98f4917cae976e7b47b56aa02836fb028dcc6..b48d79f1ba4fb89cae3e3050bfb00a62895eaef2 100644 Binary files a/assays/SynComInvasionExperiments/isa.assay.xlsx and b/assays/SynComInvasionExperiments/isa.assay.xlsx differ