From af95ae5bd78b27b072cb677f6277a22dfa4c8633 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 10:36:12 +0200
Subject: [PATCH 01/18] move img to publication folder

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rename to _publication/Wippel_2021.png
-- 
GitLab


From 38015db49400fb0c8c31deb85174fb86e32970ce Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 10:46:51 +0200
Subject: [PATCH 02/18] add more information in README

---
 README.md | 14 ++++++++++----
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diff --git a/README.md b/README.md
index 4b4bd93..baf43c5 100644
--- a/README.md
+++ b/README.md
@@ -1,19 +1,25 @@
-# Abstract for Wippel (2021)
+# Host preference and invasiveness of commensal bacteria in the *Lotus* and *Arabidopsis* root microbiota
+
+## Abstract for Wippel (2021)
 
 Roots of different plant species are colonized by bacterial communities, that are distinct even when hosts share the same habitat. It remains unclear to what extent the host actively selects these communities and whether commensals are adapted to a specific plant species. To address this question, we assembled a sequence-indexed bacterial culture collection from roots and nodules of *Lotus japonicus* that contains representatives of most species previously identified using metagenomics. We analysed taxonomically paired synthetic communities from *L. japonicus* and *Arabidopsis thaliana* in a multi-species gnotobiotic system and detected signatures of host preference among commensal bacteria in a community context, but not in mono-associations. Sequential inoculation experiments revealed priority effects during root microbiota assembly, where established communities are resilient to invasion by latecomers, and that host preference of commensal bacteria confers a competitive advantage in their cognate host. Our findings show that host preference in commensal bacteria from diverse taxonomic groups is associated with their invasiveness into standing root-associated communities.
 
-![Wippel_2021](Wippel_2021.png)
+<img src=./_publication/Wippel_2021.png width=60%>
 
 ## Publication Details
 
 - **DOI**: [10.1038/s41564-021-00941-9](https://doi.org/10.1038/s41564-021-00941-9)
-- **Title**: Host preference and invasiveness of commensal bacteria in the Lotus and Arabidopsis root microbiota
+- **Title**: Host preference and invasiveness of commensal bacteria in the *Lotus* and *Arabidopsis* root microbiota
 - **Journal**: Nature Microbiology
 - **Published on**: 2021-07-26
 
 ## Data Availability
 
-<Data availability details (if provided by the article)>
+The strains of the *Lj*-SPHERE collection will be deposited at and will be available on request from the Leibniz Institute DSMZ in Braunschweig, Germany. Raw 16S rRNA amplicon reads have been deposited in the European Nucleotide Archive under the accession number PRJEB37695. Similarly, sequencing reads and genome assemblies of the *Lj*-SPHERE core collection have been uploaded to the same database with the accession number PRJEB37696. Source data are provided with this paper.
+
+## Code availability
+
+The scripts used for the computational analyses described in this study are available at http://www.github.com/garridoo/ljsphere, to ensure replicability and reproducibility of these results.
 
 ## License
 
-- 
GitLab


From fe8b2018eb53a819213bfe89c43541663ee73c70 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 10:49:37 +0200
Subject: [PATCH 03/18] crop img

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literal 0
HcmV?d00001

-- 
GitLab


From 7430cffd399dccfd84228a621b7e8e87c2f3a481 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 10:50:27 +0200
Subject: [PATCH 04/18] fix img in README

---
 README.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/README.md b/README.md
index baf43c5..a163e51 100644
--- a/README.md
+++ b/README.md
@@ -4,7 +4,7 @@
 
 Roots of different plant species are colonized by bacterial communities, that are distinct even when hosts share the same habitat. It remains unclear to what extent the host actively selects these communities and whether commensals are adapted to a specific plant species. To address this question, we assembled a sequence-indexed bacterial culture collection from roots and nodules of *Lotus japonicus* that contains representatives of most species previously identified using metagenomics. We analysed taxonomically paired synthetic communities from *L. japonicus* and *Arabidopsis thaliana* in a multi-species gnotobiotic system and detected signatures of host preference among commensal bacteria in a community context, but not in mono-associations. Sequential inoculation experiments revealed priority effects during root microbiota assembly, where established communities are resilient to invasion by latecomers, and that host preference of commensal bacteria confers a competitive advantage in their cognate host. Our findings show that host preference in commensal bacteria from diverse taxonomic groups is associated with their invasiveness into standing root-associated communities.
 
-<img src=./_publication/Wippel_2021.png width=60%>
+<img src=./_publication/Wippel_2021_cropped.png width=60%>
 
 ## Publication Details
 
-- 
GitLab


From 48f0e083aa048cac7f4fa112befa57334d59de12 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 08:55:42 +0000
Subject: [PATCH 05/18] Add LICENSE

---
 LICENSE | 395 ++++++++++++++++++++++++++++++++++++++++++++++++++++++++
 1 file changed, 395 insertions(+)
 create mode 100644 LICENSE

diff --git a/LICENSE b/LICENSE
new file mode 100644
index 0000000..9965cb9
--- /dev/null
+++ b/LICENSE
@@ -0,0 +1,395 @@
+Attribution 4.0 International
+
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+     closely related to copyright including, without limitation,
+     performance, broadcast, sound recording, and Sui Generis Database
+     Rights, without regard to how the rights are labeled or
+     categorized. For purposes of this Public License, the rights
+     specified in Section 2(b)(1)-(2) are not Copyright and Similar
+     Rights.
+
+  d. Effective Technological Measures means those measures that, in the
+     absence of proper authority, may not be circumvented under laws
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+  e. Exceptions and Limitations means fair use, fair dealing, and/or
+     any other exception or limitation to Copyright and Similar Rights
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+  f. Licensed Material means the artistic or literary work, database,
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+     Licensed Material and that the Licensor has authority to license.
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+  h. Licensor means the individual(s) or entity(ies) granting rights
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+     available to the public including in ways that members of the
+     public may access the material from a place and at a time
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+
+  j. Sui Generis Database Rights means rights other than copyright
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+     the Council of 11 March 1996 on the legal protection of databases,
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+     equivalent rights anywhere in the world.
+
+  k. You means the individual or entity exercising the Licensed Rights
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+
+
+Section 2 -- Scope.
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+  a. License grant.
+
+       1. Subject to the terms and conditions of this Public License,
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+          exercise the Licensed Rights in the Licensed Material to:
+
+            a. reproduce and Share the Licensed Material, in whole or
+               in part; and
+
+            b. produce, reproduce, and Share Adapted Material.
+
+       2. Exceptions and Limitations. For the avoidance of doubt, where
+          Exceptions and Limitations apply to Your use, this Public
+          License does not apply, and You do not need to comply with
+          its terms and conditions.
+
+       3. Term. The term of this Public License is specified in Section
+          6(a).
+
+       4. Media and formats; technical modifications allowed. The
+          Licensor authorizes You to exercise the Licensed Rights in
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+          Licensor waives and/or agrees not to assert any right or
+          authority to forbid You from making technical modifications
+          necessary to exercise the Licensed Rights, including
+          technical modifications necessary to circumvent Effective
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+          simply making modifications authorized by this Section 2(a)
+          (4) never produces Adapted Material.
+
+       5. Downstream recipients.
+
+            a. Offer from the Licensor -- Licensed Material. Every
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+            b. No downstream restrictions. You may not offer or impose
+               any additional or different terms or conditions on, or
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+               Licensed Rights by any recipient of the Licensed
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+       6. No endorsement. Nothing in this Public License constitutes or
+          may be construed as permission to assert or imply that You
+          are, or that Your use of the Licensed Material is, connected
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+          provided in Section 3(a)(1)(A)(i).
+
+  b. Other rights.
+
+       1. Moral rights, such as the right of integrity, are not
+          licensed under this Public License, nor are publicity,
+          privacy, and/or other similar personality rights; however, to
+          the extent possible, the Licensor waives and/or agrees not to
+          assert any such rights held by the Licensor to the limited
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+
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+       3. To the extent possible, the Licensor waives any right to
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+
+Section 3 -- License Conditions.
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+Your exercise of the Licensed Rights is expressly made subject to the
+following conditions.
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+
+       1. If You Share the Licensed Material (including in modified
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+
+            a. retain the following if it is supplied by the Licensor
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+
+                iv. a notice that refers to the disclaimer of
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+
+            c. indicate the Licensed Material is licensed under this
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+               hyperlink to, this Public License.
+
+       2. You may satisfy the conditions in Section 3(a)(1) in any
+          reasonable manner based on the medium, means, and context in
+          which You Share the Licensed Material. For example, it may be
+          reasonable to satisfy the conditions by providing a URI or
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+          information.
+
+       3. If requested by the Licensor, You must remove any of the
+          information required by Section 3(a)(1)(A) to the extent
+          reasonably practicable.
+
+       4. If You Share Adapted Material You produce, the Adapter's
+          License You apply must not prevent recipients of the Adapted
+          Material from complying with this Public License.
+
+
+Section 4 -- Sui Generis Database Rights.
+
+Where the Licensed Rights include Sui Generis Database Rights that
+apply to Your use of the Licensed Material:
+
+  a. for the avoidance of doubt, Section 2(a)(1) grants You the right
+     to extract, reuse, reproduce, and Share all or a substantial
+     portion of the contents of the database;
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+     Rights, then the database in which You have Sui Generis Database
+     Rights (but not its individual contents) is Adapted Material; and
+
+  c. You must comply with the conditions in Section 3(a) if You Share
+     all or a substantial portion of the contents of the database.
+
+For the avoidance of doubt, this Section 4 supplements and does not
+replace Your obligations under this Public License where the Licensed
+Rights include other Copyright and Similar Rights.
+
+
+Section 5 -- Disclaimer of Warranties and Limitation of Liability.
+
+  a. UNLESS OTHERWISE SEPARATELY UNDERTAKEN BY THE LICENSOR, TO THE
+     EXTENT POSSIBLE, THE LICENSOR OFFERS THE LICENSED MATERIAL AS-IS
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+
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+     TO YOU ON ANY LEGAL THEORY (INCLUDING, WITHOUT LIMITATION,
+     NEGLIGENCE) OR OTHERWISE FOR ANY DIRECT, SPECIAL, INDIRECT,
+     INCIDENTAL, CONSEQUENTIAL, PUNITIVE, EXEMPLARY, OR OTHER LOSSES,
+     COSTS, EXPENSES, OR DAMAGES ARISING OUT OF THIS PUBLIC LICENSE OR
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+     ADVISED OF THE POSSIBILITY OF SUCH LOSSES, COSTS, EXPENSES, OR
+     DAMAGES. WHERE A LIMITATION OF LIABILITY IS NOT ALLOWED IN FULL OR
+     IN PART, THIS LIMITATION MAY NOT APPLY TO YOU.
+
+  c. The disclaimer of warranties and limitation of liability provided
+     above shall be interpreted in a manner that, to the extent
+     possible, most closely approximates an absolute disclaimer and
+     waiver of all liability.
+
+
+Section 6 -- Term and Termination.
+
+  a. This Public License applies for the term of the Copyright and
+     Similar Rights licensed here. However, if You fail to comply with
+     this Public License, then Your rights under this Public License
+     terminate automatically.
+
+  b. Where Your right to use the Licensed Material has terminated under
+     Section 6(a), it reinstates:
+
+       1. automatically as of the date the violation is cured, provided
+          it is cured within 30 days of Your discovery of the
+          violation; or
+
+       2. upon express reinstatement by the Licensor.
+
+     For the avoidance of doubt, this Section 6(b) does not affect any
+     right the Licensor may have to seek remedies for Your violations
+     of this Public License.
+
+  c. For the avoidance of doubt, the Licensor may also offer the
+     Licensed Material under separate terms or conditions or stop
+     distributing the Licensed Material at any time; however, doing so
+     will not terminate this Public License.
+
+  d. Sections 1, 5, 6, 7, and 8 survive termination of this Public
+     License.
+
+
+Section 7 -- Other Terms and Conditions.
+
+  a. The Licensor shall not be bound by any additional or different
+     terms or conditions communicated by You unless expressly agreed.
+
+  b. Any arrangements, understandings, or agreements regarding the
+     Licensed Material not stated herein are separate from and
+     independent of the terms and conditions of this Public License.
+
+
+Section 8 -- Interpretation.
+
+  a. For the avoidance of doubt, this Public License does not, and
+     shall not be interpreted to, reduce, limit, restrict, or impose
+     conditions on any use of the Licensed Material that could lawfully
+     be made without permission under this Public License.
+
+  b. To the extent possible, if any provision of this Public License is
+     deemed unenforceable, it shall be automatically reformed to the
+     minimum extent necessary to make it enforceable. If the provision
+     cannot be reformed, it shall be severed from this Public License
+     without affecting the enforceability of the remaining terms and
+     conditions.
+
+  c. No term or condition of this Public License will be waived and no
+     failure to comply consented to unless expressly agreed to by the
+     Licensor.
+
+  d. Nothing in this Public License constitutes or may be interpreted
+     as a limitation upon, or waiver of, any privileges and immunities
+     that apply to the Licensor or You, including from the legal
+     processes of any jurisdiction or authority.
+
+
+=======================================================================
+
+Creative Commons is not a party to its public licenses.
+Notwithstanding, Creative Commons may elect to apply one of its public
+licenses to material it publishes and in those instances will be
+considered the “Licensor.” The text of the Creative Commons public
+licenses is dedicated to the public domain under the CC0 Public Domain
+Dedication. Except for the limited purpose of indicating that material
+is shared under a Creative Commons public license or as otherwise
+permitted by the Creative Commons policies published at
+creativecommons.org/policies, Creative Commons does not authorize the
+use of the trademark "Creative Commons" or any other trademark or logo
+of Creative Commons without its prior written consent including,
+without limitation, in connection with any unauthorized modifications
+to any of its public licenses or any other arrangements,
+understandings, or agreements concerning use of licensed material. For
+the avoidance of doubt, this paragraph does not form part of the public
+licenses.
+
+Creative Commons may be contacted at creativecommons.org.
\ No newline at end of file
-- 
GitLab


From ea7d9913349a2fb1470a4f5e80ca5ded56e3183e Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 11:26:37 +0200
Subject: [PATCH 06/18] add extended data

---
 .../ExtendedData/41564_2021_941_Fig10_ESM.webp | Bin 0 -> 228884 bytes
 .../ExtendedData/41564_2021_941_Fig11_ESM.webp | Bin 0 -> 328590 bytes
 .../ExtendedData/41564_2021_941_Fig12_ESM.webp | Bin 0 -> 120748 bytes
 .../ExtendedData/41564_2021_941_Fig13_ESM.webp | Bin 0 -> 226728 bytes
 .../ExtendedData/41564_2021_941_Fig14_ESM.webp | Bin 0 -> 62718 bytes
 .../ExtendedData/41564_2021_941_Fig15_ESM.webp | Bin 0 -> 185778 bytes
 .../ExtendedData/41564_2021_941_Fig16_ESM.webp | Bin 0 -> 93182 bytes
 .../ExtendedData/41564_2021_941_Fig7_ESM.webp  | Bin 0 -> 268958 bytes
 .../ExtendedData/41564_2021_941_Fig8_ESM.webp  | Bin 0 -> 121086 bytes
 .../ExtendedData/41564_2021_941_Fig9_ESM.webp  | Bin 0 -> 86960 bytes
 10 files changed, 0 insertions(+), 0 deletions(-)
 create mode 100644 _publication/ExtendedData/41564_2021_941_Fig10_ESM.webp
 create mode 100644 _publication/ExtendedData/41564_2021_941_Fig11_ESM.webp
 create mode 100644 _publication/ExtendedData/41564_2021_941_Fig12_ESM.webp
 create mode 100644 _publication/ExtendedData/41564_2021_941_Fig13_ESM.webp
 create mode 100644 _publication/ExtendedData/41564_2021_941_Fig14_ESM.webp
 create mode 100644 _publication/ExtendedData/41564_2021_941_Fig15_ESM.webp
 create mode 100644 _publication/ExtendedData/41564_2021_941_Fig16_ESM.webp
 create mode 100644 _publication/ExtendedData/41564_2021_941_Fig7_ESM.webp
 create mode 100644 _publication/ExtendedData/41564_2021_941_Fig8_ESM.webp
 create mode 100644 _publication/ExtendedData/41564_2021_941_Fig9_ESM.webp

diff --git a/_publication/ExtendedData/41564_2021_941_Fig10_ESM.webp b/_publication/ExtendedData/41564_2021_941_Fig10_ESM.webp
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HcmV?d00001

-- 
GitLab


From f9e172b91766934fc53a52ded13de08ca6ef7caf Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 11:27:58 +0200
Subject: [PATCH 07/18] add README for extended data

---
 _publication/ExtendedData/README.md | 51 +++++++++++++++++++++++++++++
 1 file changed, 51 insertions(+)
 create mode 100644 _publication/ExtendedData/README.md

diff --git a/_publication/ExtendedData/README.md b/_publication/ExtendedData/README.md
new file mode 100644
index 0000000..0702a3e
--- /dev/null
+++ b/_publication/ExtendedData/README.md
@@ -0,0 +1,51 @@
+# Extended Data
+
+### Extended Data Fig. 1: Flow chart of experimental procedures.
+**a**, Overview of experiments and type of analyses performed on root, rhizosphere, and soil samples, or millifluidic droplets. **b**, Culture collection establishment of *Lotus japonicus* root and nodule bacterial isolates. Image created with BioRender.com.
+
+https://www.nature.com/articles/s41564-021-00941-9/figures/7
+
+### Extended Data Fig. 2 Culture-independent diversity analysis of root-associated bacterial communities from *Lotus* and *Arabidopsis*.
+Rank abundance plot of bacterial communities from *Lotus* or *Arabidopsis* roots, aggregated to the class level, including all families with a mean accumulated relative abundance of > 0.1% on either host. Statistical differences were assessed using a two-sided, non-parametric Mann–Whitney test. Asterisks represent significant values after multiple testing correction using the Benjamini–Hochberg method (*P* < 0.05).
+
+https://www.nature.com/articles/s41564-021-00941-9/figures/8
+
+### Extended Data Fig. 3 Functional overlap of *Lotus* and *Arabidopsis* culture collection genomes.
+PCoA of functional distances of genomes from bacterial isolates of the *Lotus* (*Lj*-SPHERE; n = 294) and the *Arabidopsis* (*At*-SPHERE; n = 194) culture collections.
+
+https://www.nature.com/articles/s41564-021-00941-9/figures/9
+
+### Extended Data Fig. 4 Host-species specific bacterial root communities and commensal host preference is confirmed using independent mixed communities.
+**a** and **c**, Constrained PCoA of Bray-Curtis dissimilarity (constrained by all biological factors and conditioned by all technical variables) of soil, rhizosphere, and root samples from *L. japonicus* wild-type Gifu, *nfr5* mutant, and *A. thaliana* wild type Col-0 plants co-cultivated with the mixed community *LjAt*-SC1 (**a**, experiment C, n = 155, variance explained 53.8%, *P* = 0.001), from Gifu and Col-0 co-cultivated with *LjAt*-SC4 (**b**, experiment D, n = 87, variance explained 60%, *P* = 0.001), or from Gifu and Col-0 co-cultivated with *LjAt*-SC5 (**b**, experiment M, n = 100, variance explained 67%, *P* = 0.001). **d**, **e** and **f**, Aggregated RA of the 16 *Lj*-derived and the 16 *At*-derived strains in the roots of *Lotus* and *Arabidopsis* plants inoculated with *LjAt*-SC1 (**d**; n = 68), *LjAt*-SC4 (**e**; n = 34), or *LjAt*-SC5 (**f**; n = 40). n refers to biologically independent samples.
+
+https://www.nature.com/articles/s41564-021-00941-9/figures/10
+
+### Extended Data Fig. 5 Host preference is retained after in silico removal of individual bacterial families.
+Aggregated relative abundance of *Lotus*- (**a**, n = 20) and *Arabidopsis*-derived (**b**, n = 16) strains in roots from plants inoculated with the mixed community *LjAt*-SC3 (experiment L). Host preference was assessed using a Mann-Whitney non-parametric test after *in silico* removal of each family. The x-axis labels indicate each depleted family. n refers to biologically independent samples.
+
+https://www.nature.com/articles/s41564-021-00941-9/figures/11
+
+### Extended Data Fig. 6 Bacterial abundance in mono-association with host plants.
+Bacterial abundances of commensal bacteria (strain IDs indicated at x-axis) colonizing Col-0, Gifu, and *nfr5* roots, assessed by counting of colony forming units (CFUs) after extraction from root tissue. Plants were grown for two weeks on agar plates in mono-association with the indicated Lj-SPHERE (**a**) and At-SPHERE (**b**) strains (exp. E). n = 6 (3 biologically independent samples × 2 technical replicates). Statistical differences were assessed using a two-sided, non-parametric Mann-Whitney test. Asterisks represent significant values after multiple testing correction using the Benjamini–Hochberg method (*P* < 0.05).
+
+https://www.nature.com/articles/s41564-021-00941-9/figures/12
+
+### Extended Data Fig. 7 Plant performance in mono-associations.
+Shoot fresh weight of Gifu (**a**) and Col-0 (**b**) plants grown for two weeks on agar plates in mono-association with the indicated *Lj*-SPHERE and *At*-SPHERE strains (exp. E). n = 30 (3 biologically independent samples × 10 technical replicates).
+
+https://www.nature.com/articles/s41564-021-00941-9/figures/13
+
+### Extended Data Fig. 8 Sister species of *L. japonicus* and *A. thaliana* establish distinct bacterial root communities.
+Constrained PCoA of Bray-Curtis dissimilarity (constrained by all biological factors and conditioned by all technical variables) of root samples from *L. japonicus* wild type Gifu and *L. corniculatus* (**a**; n = 87; variance explained 58.7%, *P* = 0.001), and or root samples from *A. thaliana* wild type Col-0 and *A. lyrata* MN47 (**b**; n = 86; variance explained 65%, *P* = 0.001), inoculated and grown with the mixed community *LjAt*-SC3, and of the corresponding rhizosphere and bulk soil communities (exp. F).
+
+https://www.nature.com/articles/s41564-021-00941-9/figures/14
+
+### Extended Data Fig. 9 Tested plant immune receptors and signaling pathways do not affect host preference of commensals.
+**a**, Constrained PCoA of Bray-Curtis dissimilarity (constrained by all biological factors and conditioned by all technical variables; n = 98; variance explained 24.6%, *P* = 0.001) of root samples from *L. japonicus* wild type Gifu, *Ljfls2* mutant, *A. thaliana* wild type Col-0, *Atfls2* mutant, and *Atbbc* mutant inoculated and grown with the mixed SynCom *LjAt*-SC1 (exp. G), and of the corresponding bacterial input communities. **b**, Aggregated relative abundance of the 16 *Lj*-derived and the 16 *At*-derived strains in the roots of *Lotus* and *Arabidopsis* plants. n = 21 for Gifu, n = 16 for Col-0, n = 20 for *Ljfls2* and *Atfls2*, n = 18 for *Atbbc*. **c**, Constrained PCoA of Bray-Curtis dissimilarity (constrained by all biological factors and conditioned by all technical variables; n = 64; variance explained 42.2%, *P* = 0.001) of soil and root samples from Gifu, Col-0, and *Atdeps* mutant inoculated and grown with the mixed SynCom *LjAt*-SC3 (exp. H). **d**, Aggregated relative abundance of the 16 *Lj*-derived and the 16 *At*-derived strains in the roots of *Lotus* and *Arabidopsis* plants. n = 14 for Gifu, n = 20 for Col-0 and *Atdeps*. n refers to biologically independent samples.
+
+https://www.nature.com/articles/s41564-021-00941-9/figures/15
+
+### Extended Data Fig. 10 Effect of secreted indole glucosinolates on host preference of commensals.
+**a**, Constrained PCoA of Bray-Curtis dissimilarity (constrained by all biological factors and conditioned by all technical variables; n = 50; variance explained 47.2%, *P* = 0.001) of soil and root samples from *L. japonicus* wild type Gifu, *A. thaliana* wild type Col-0, and *Arabidopsis cyp79b2 cyb79b3* mutant inoculated and grown with the mixed SynCom *LjAt*-SC3 (exp. H). **b**, Aggregated relative abundance of the 16 *Lj*-derived and the 16 *At*-derived strains in the roots of *Lotus* and *Arabidopsis* plants. n = 20 for Col-0, n = 14 for Gifu, n = 6 for *Atcyp79b2b3*. n refers to biologically independent samples.
+
+https://www.nature.com/articles/s41564-021-00941-9/figures/16
\ No newline at end of file
-- 
GitLab


From 73c91279c0611dd8f609acdf5bfedfe00845a382 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 11:36:09 +0200
Subject: [PATCH 08/18] upload supplementary information files

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C?8(#s

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HcmV?d00001

-- 
GitLab


From ac4774a1b341f93b2662c1f1383b22a417686a35 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 11:38:12 +0200
Subject: [PATCH 09/18] add README for supplementary information

---
 .../SupplementaryInformation/README.md        | 25 +++++++++++++++++++
 1 file changed, 25 insertions(+)
 create mode 100644 _publication/SupplementaryInformation/README.md

diff --git a/_publication/SupplementaryInformation/README.md b/_publication/SupplementaryInformation/README.md
new file mode 100644
index 0000000..ea98a2a
--- /dev/null
+++ b/_publication/SupplementaryInformation/README.md
@@ -0,0 +1,25 @@
+# Supplementary Information
+
+### Supplementary Information
+Supplementary Note, Figs. 1–3 and Tables 1 and 2.
+
+https://static-content.springer.com/esm/art%3A10.1038%2Fs41564-021-00941-9/MediaObjects/41564_2021_941_MOESM1_ESM.pdf
+
+### Reporting Summary
+
+https://static-content.springer.com/esm/art%3A10.1038%2Fs41564-021-00941-9/MediaObjects/41564_2021_941_MOESM2_ESM.pdf
+
+### Supplementary Data 1
+Data and metadata of *Lj*- and *At*-IRLs.
+
+https://static-content.springer.com/esm/art%3A10.1038%2Fs41564-021-00941-9/MediaObjects/41564_2021_941_MOESM3_ESM.zip
+
+### Supplementary Data 2
+Metadata of the *Lj*- and *At*-SPHERE core culture collection.
+
+https://static-content.springer.com/esm/art%3A10.1038%2Fs41564-021-00941-9/MediaObjects/41564_2021_941_MOESM4_ESM.zip
+
+### Supplementary Data 3
+Data and metadata of *LjAt* SynCom experiments.
+
+https://static-content.springer.com/esm/art%3A10.1038%2Fs41564-021-00941-9/MediaObjects/41564_2021_941_MOESM5_ESM.zip
\ No newline at end of file
-- 
GitLab


From 98bfd7f65da931448c610b3ed5900f8d2b4ac4e2 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 11:42:05 +0200
Subject: [PATCH 10/18] upload source data

---
 .gitattributes                                 |   2 ++
 .../SourceData/41564_2021_941_MOESM10_ESM.zip  | Bin 0 -> 68131 bytes
 .../SourceData/41564_2021_941_MOESM11_ESM.zip  | Bin 0 -> 33807 bytes
 .../SourceData/41564_2021_941_MOESM6_ESM.zip   |   3 +++
 .../SourceData/41564_2021_941_MOESM7_ESM.zip   | Bin 0 -> 13470 bytes
 .../SourceData/41564_2021_941_MOESM8_ESM.zip   | Bin 0 -> 141989 bytes
 .../SourceData/41564_2021_941_MOESM9_ESM.zip   |   3 +++
 7 files changed, 8 insertions(+)
 create mode 100644 _publication/SourceData/41564_2021_941_MOESM10_ESM.zip
 create mode 100644 _publication/SourceData/41564_2021_941_MOESM11_ESM.zip
 create mode 100644 _publication/SourceData/41564_2021_941_MOESM6_ESM.zip
 create mode 100644 _publication/SourceData/41564_2021_941_MOESM7_ESM.zip
 create mode 100644 _publication/SourceData/41564_2021_941_MOESM8_ESM.zip
 create mode 100644 _publication/SourceData/41564_2021_941_MOESM9_ESM.zip

diff --git a/.gitattributes b/.gitattributes
index 583ef93..a4fca86 100644
--- a/.gitattributes
+++ b/.gitattributes
@@ -1,3 +1,5 @@
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 _publication/SupplementaryInformation/41564_2021_941_MOESM3_ESM.zip filter=lfs diff=lfs merge=lfs -text
+_publication/SourceData/41564_2021_941_MOESM6_ESM.zip filter=lfs diff=lfs merge=lfs -text
+_publication/SourceData/41564_2021_941_MOESM9_ESM.zip filter=lfs diff=lfs merge=lfs -text
diff --git a/_publication/SourceData/41564_2021_941_MOESM10_ESM.zip b/_publication/SourceData/41564_2021_941_MOESM10_ESM.zip
new file mode 100644
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diff --git a/_publication/SourceData/41564_2021_941_MOESM9_ESM.zip b/_publication/SourceData/41564_2021_941_MOESM9_ESM.zip
new file mode 100644
index 0000000..a71bbfa
--- /dev/null
+++ b/_publication/SourceData/41564_2021_941_MOESM9_ESM.zip
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+oid sha256:c737bed8dd3213d8888411f8bf3b333b7823d0af04c42db93774efb789674560
+size 17584385
-- 
GitLab


From d2de5d3ea62f54103f8481e558833d97032d5adc Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 11:43:26 +0200
Subject: [PATCH 11/18] add README  for source data

---
 _publication/SourceData/README.md | 31 +++++++++++++++++++++++++++++++
 1 file changed, 31 insertions(+)
 create mode 100644 _publication/SourceData/README.md

diff --git a/_publication/SourceData/README.md b/_publication/SourceData/README.md
new file mode 100644
index 0000000..d67c7be
--- /dev/null
+++ b/_publication/SourceData/README.md
@@ -0,0 +1,31 @@
+# Source Data
+
+### Source Data Fig. 1
+Statistical source data.
+
+https://static-content.springer.com/esm/art%3A10.1038%2Fs41564-021-00941-9/MediaObjects/41564_2021_941_MOESM6_ESM.zip
+
+### Source Data Fig. 2
+Statistical source data.
+
+https://static-content.springer.com/esm/art%3A10.1038%2Fs41564-021-00941-9/MediaObjects/41564_2021_941_MOESM7_ESM.zip
+
+### Source Data Fig. 3
+Statistical source data.
+
+https://static-content.springer.com/esm/art%3A10.1038%2Fs41564-021-00941-9/MediaObjects/41564_2021_941_MOESM8_ESM.zip
+
+### Source Data Fig. 4
+Statistical source data.
+
+https://static-content.springer.com/esm/art%3A10.1038%2Fs41564-021-00941-9/MediaObjects/41564_2021_941_MOESM9_ESM.zip
+
+### Source Data Fig. 5
+Statistical source data.
+
+https://static-content.springer.com/esm/art%3A10.1038%2Fs41564-021-00941-9/MediaObjects/41564_2021_941_MOESM10_ESM.zip
+
+### Source Data Fig. 6
+Statistical source data.
+
+https://static-content.springer.com/esm/art%3A10.1038%2Fs41564-021-00941-9/MediaObjects/41564_2021_941_MOESM11_ESM.zip
\ No newline at end of file
-- 
GitLab


From 2bb4909acabf03dfeeb05e5ec9186bda07fa26bb Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 11:56:53 +0200
Subject: [PATCH 12/18] fill in isa investigation

---
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-- 
GitLab


From 0056f2090e602b2adc70407907c856c782439f11 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 12:19:31 +0200
Subject: [PATCH 13/18] add a study

---
 .../LotusJaponicusCultureCollection/README.md    |   0
 .../isa.study.xlsx                               | Bin 0 -> 7509 bytes
 .../protocols/.gitkeep                           |   0
 .../resources/.gitkeep                           |   0
 4 files changed, 0 insertions(+), 0 deletions(-)
 create mode 100644 studies/LotusJaponicusCultureCollection/README.md
 create mode 100644 studies/LotusJaponicusCultureCollection/isa.study.xlsx
 create mode 100644 studies/LotusJaponicusCultureCollection/protocols/.gitkeep
 create mode 100644 studies/LotusJaponicusCultureCollection/resources/.gitkeep

diff --git a/studies/LotusJaponicusCultureCollection/README.md b/studies/LotusJaponicusCultureCollection/README.md
new file mode 100644
index 0000000..e69de29
diff --git a/studies/LotusJaponicusCultureCollection/isa.study.xlsx b/studies/LotusJaponicusCultureCollection/isa.study.xlsx
new file mode 100644
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diff --git a/studies/LotusJaponicusCultureCollection/protocols/.gitkeep b/studies/LotusJaponicusCultureCollection/protocols/.gitkeep
new file mode 100644
index 0000000..e69de29
diff --git a/studies/LotusJaponicusCultureCollection/resources/.gitkeep b/studies/LotusJaponicusCultureCollection/resources/.gitkeep
new file mode 100644
index 0000000..e69de29
-- 
GitLab


From 1026ba67d1c89c4371ca84c45291a7e29560828b Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 13:22:53 +0200
Subject: [PATCH 14/18] upload first batch of rna-seq files

---
 .gitattributes                                |  32 ++++++++++++++++++
 assays/Lj-SPHERE-rRNA-seq/README.md           |   0
 assays/Lj-SPHERE-rRNA-seq/dataset/.gitkeep    |   0
 .../dataset/LjAt_001_CR.p1.1.fastq.gz         |   3 ++
 .../dataset/LjAt_001_CRh.p1.4.fastq.gz        |   3 ++
 .../dataset/LjAt_001_gR.p2.8.fastq.gz         |   3 ++
 .../dataset/LjAt_001_np.p1.7.fastq.gz         |   3 ++
 .../dataset/LjAt_002_A48.fastq.gz             |   3 ++
 .../dataset/LjAt_002_L23.fastq.gz             |   3 ++
 .../dataset/LjAt_002_L41.fastq.gz             |   3 ++
 .../dataset/LjAt_002_L5.fastq.gz              |   3 ++
 .../dataset/LjAt_002_N5.fastq.gz              |   3 ++
 .../dataset/LjAt_004_AA16.R.fastq.gz          |   3 ++
 .../dataset/LjAt_004_AF17.H.fastq.gz          |   3 ++
 .../dataset/LjAt_004_LA13.R.fastq.gz          |   3 ++
 .../dataset/LjAt_004_LA9.R.fastq.gz           |   3 ++
 .../dataset/LjAt_005_Gifu.19.fastq.gz         |   3 ++
 .../dataset/LjAt_005_deps.7.fastq.gz          |   3 ++
 .../dataset/LjAt_006_AF12R.fastq.gz           |   3 ++
 .../dataset/LjAt_006_LA10R.fastq.gz           |   3 ++
 .../dataset/LjAt_006_LA3R.fastq.gz            |   3 ++
 .../dataset/LjAt_006_LA8R.fastq.gz            |   3 ++
 .../dataset/LjAt_006_LF3.fastq.gz             |   3 ++
 .../dataset/LjAt_006_LL14.fastq.gz            |   3 ++
 .../dataset/LjAt_006_LL2R.fastq.gz            |   3 ++
 .../dataset/LjAt_006_LL7R.fastq.gz            |   3 ++
 .../dataset/LjAt_006_NL6a.fastq.gz            |   3 ++
 .../LjAt_007_LjAt.007.At19.19.root.fastq.gz   |   3 ++
 .../dataset/LjAt_008_At5.9.R.fastq.gz         |   3 ++
 .../dataset/LjAt_008_Lj12.9.R.fastq.gz        |   3 ++
 .../dataset/LjAt_008_Lj19.4.R.fastq.gz        |   3 ++
 .../dataset/LjAt_008_np12.11.fastq.gz         |   3 ++
 .../dataset/LjAt_009_At10R.fastq.gz           |   3 ++
 .../dataset/LjAt_009_Lj10.fastq.gz            |   3 ++
 .../dataset/LjAt_009_Lj11.fastq.gz            |   3 ++
 .../dataset/LjAt_009_Lj1R.fastq.gz            |   3 ++
 .../dataset/LjAt_010_B17.fastq.gz             |   3 ++
 .../dataset/LjAt_010_C11.fastq.gz             |   3 ++
 .../dataset/LjAt_010_E9.fastq.gz              |   3 ++
 .../dataset/LjAt_010_F3.fastq.gz              |   3 ++
 .../dataset/MDA10_LA.Col0.2.fastq.gz          |   3 ++
 .../dataset/MDA10_LA.Gifu.4.fastq.gz          |   3 ++
 .../dataset/MDA10_LA.nfr5.5.fastq.gz          |   3 ++
 assays/Lj-SPHERE-rRNA-seq/isa.assay.xlsx      | Bin 0 -> 6902 bytes
 assays/Lj-SPHERE-rRNA-seq/protocols/.gitkeep  |   0
 45 files changed, 152 insertions(+)
 create mode 100644 assays/Lj-SPHERE-rRNA-seq/README.md
 create mode 100644 assays/Lj-SPHERE-rRNA-seq/dataset/.gitkeep
 create mode 100644 assays/Lj-SPHERE-rRNA-seq/dataset/LjAt_001_CR.p1.1.fastq.gz
 create mode 100644 assays/Lj-SPHERE-rRNA-seq/dataset/LjAt_001_CRh.p1.4.fastq.gz
 create mode 100644 assays/Lj-SPHERE-rRNA-seq/dataset/LjAt_001_gR.p2.8.fastq.gz
 create mode 100644 assays/Lj-SPHERE-rRNA-seq/dataset/LjAt_001_np.p1.7.fastq.gz
 create mode 100644 assays/Lj-SPHERE-rRNA-seq/dataset/LjAt_002_A48.fastq.gz
 create mode 100644 assays/Lj-SPHERE-rRNA-seq/dataset/LjAt_002_L23.fastq.gz
 create mode 100644 assays/Lj-SPHERE-rRNA-seq/dataset/LjAt_002_L41.fastq.gz
 create mode 100644 assays/Lj-SPHERE-rRNA-seq/dataset/LjAt_002_L5.fastq.gz
 create mode 100644 assays/Lj-SPHERE-rRNA-seq/dataset/LjAt_002_N5.fastq.gz
 create mode 100644 assays/Lj-SPHERE-rRNA-seq/dataset/LjAt_004_AA16.R.fastq.gz
 create mode 100644 assays/Lj-SPHERE-rRNA-seq/dataset/LjAt_004_AF17.H.fastq.gz
 create mode 100644 assays/Lj-SPHERE-rRNA-seq/dataset/LjAt_004_LA13.R.fastq.gz
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diff --git a/assays/Lj-SPHERE-rRNA-seq/protocols/.gitkeep b/assays/Lj-SPHERE-rRNA-seq/protocols/.gitkeep
new file mode 100644
index 0000000..e69de29
-- 
GitLab


From 1c04ff200a7e1aea654cede06c4059842c1c554e Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 13:28:12 +0200
Subject: [PATCH 15/18] upload second batch of rna-seq

---
 .gitattributes                                | 83 +++++++++++++++++++
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 .../dataset/LjAt_001_CR.p2.6.fastq.gz         |  3 +
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 .../dataset/MDA10_LA.nfr5.18.fastq.gz         |  3 +
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 108 files changed, 404 insertions(+)
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-- 
GitLab


From 9b94445194f5b9256201bb8ca2c347a75ca5fe56 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 13:40:37 +0200
Subject: [PATCH 16/18] upload 3rd batch

---
 .gitattributes                                | 167 ++++++++++++++++++
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 201 files changed, 767 insertions(+)
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diff --git a/.gitattributes b/.gitattributes
index b244f04..8585293 100644
--- a/.gitattributes
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GitLab


From ef377e9b0544ebbc656b48fd911c2af38e977203 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 14:33:03 +0200
Subject: [PATCH 17/18] sync changes via ARCCommander

---
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diff --git a/.gitattributes b/.gitattributes
index 8585293..6ff0b0d 100644
--- a/.gitattributes
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GitLab


From 616f7fd803eb3225425582ae0d8978d74f613045 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 28 Sep 2024 14:52:27 +0200
Subject: [PATCH 18/18] sync changes via ARCCommander

---
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