From 77924db11f01d09e903e8526201bbdc686c185e4 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 2 Nov 2024 14:33:10 +0100
Subject: [PATCH] add mono-associations assay and protocol
---
assays/MillifluidicsExperiment/isa.assay.xlsx | Bin 6902 -> 6902 bytes
.../README.md | 0
.../dataset/.gitkeep | 0
.../isa.assay.xlsx | Bin 0 -> 6918 bytes
.../protocols/.gitkeep | 0
...nsOfSynComMembersWithHostPlantsProtocol.md | 3 +++
6 files changed, 3 insertions(+)
create mode 100644 assays/Mono-associationsOfSynComMembersWithHostPlants/README.md
create mode 100644 assays/Mono-associationsOfSynComMembersWithHostPlants/dataset/.gitkeep
create mode 100644 assays/Mono-associationsOfSynComMembersWithHostPlants/isa.assay.xlsx
create mode 100644 assays/Mono-associationsOfSynComMembersWithHostPlants/protocols/.gitkeep
create mode 100644 assays/Mono-associationsOfSynComMembersWithHostPlants/protocols/Mono-associationsOfSynComMembersWithHostPlantsProtocol.md
diff --git a/assays/MillifluidicsExperiment/isa.assay.xlsx b/assays/MillifluidicsExperiment/isa.assay.xlsx
index ac7224fadf3244fc4f7787d9b3560f0751889fff..b26f3e3546ef53cb3360dd42044f7bcac913a8be 100644
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diff --git a/assays/Mono-associationsOfSynComMembersWithHostPlants/README.md b/assays/Mono-associationsOfSynComMembersWithHostPlants/README.md
new file mode 100644
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diff --git a/assays/Mono-associationsOfSynComMembersWithHostPlants/dataset/.gitkeep b/assays/Mono-associationsOfSynComMembersWithHostPlants/dataset/.gitkeep
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diff --git a/assays/Mono-associationsOfSynComMembersWithHostPlants/protocols/.gitkeep b/assays/Mono-associationsOfSynComMembersWithHostPlants/protocols/.gitkeep
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diff --git a/assays/Mono-associationsOfSynComMembersWithHostPlants/protocols/Mono-associationsOfSynComMembersWithHostPlantsProtocol.md b/assays/Mono-associationsOfSynComMembersWithHostPlants/protocols/Mono-associationsOfSynComMembersWithHostPlantsProtocol.md
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@@ -0,0 +1,3 @@
+## Mono-associations of SynCom members with host plants
+
+*Lotus* seeds were sterilized and placed on sterile wet Whatman paper for germination. Seedlings were transferred to squared petri dishes containing 0.25× B&D medium (with Fe-EDTA instead of Fe-citrate) supplemented with 3 mM KNO3 and 1% Difco Bacteriological agar, and sterile filter paper was put on top of the sloped solidified medium before placing the seedlings to prevent root growth inside the agar. *Arabidopsis* seeds were sterilized and germinated on 0.5× MS medium plus 1% Difco Bacteriological agar. Seedlings were transferred to squared petri dishes containing 0.5× MS medium (neutral pH, buffered with 2 mM HEPES) plus 1% agar. The 32 strains of the mixed community *LjAt*-SC3 were grown individually in liquid medium, harvested and adjusted to an OD600 of 0.02. Seedlings were inoculated by adding 500 µl of bacterial culture to the roots. Plants were grown for 14 d under long-day conditions (16/8 day–night cycles) at 21 °C. Three biological replicates were prepared for each genotype–bacteria combination.
\ No newline at end of file
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