diff --git a/assays/GreenhouseExperiment/README.md b/assays/GreenhouseExperiment/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/GreenhouseExperiment/dataset/.gitkeep b/assays/GreenhouseExperiment/dataset/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/GreenhouseExperiment/isa.assay.xlsx b/assays/GreenhouseExperiment/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..ce191913f0b646157de87ac2d0afa66f09496f14 Binary files /dev/null and b/assays/GreenhouseExperiment/isa.assay.xlsx differ diff --git a/assays/GreenhouseExperiment/protocols/.gitkeep b/assays/GreenhouseExperiment/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/GreenhouseExperiment/protocols/GreenhouseExperimentProtocol.md b/assays/GreenhouseExperiment/protocols/GreenhouseExperimentProtocol.md new file mode 100644 index 0000000000000000000000000000000000000000..66f4909f5a4649408dba45f46a8777f3c72872fd --- /dev/null +++ b/assays/GreenhouseExperiment/protocols/GreenhouseExperimentProtocol.md @@ -0,0 +1,3 @@ +## Greenhouse experiment + +*Lj* Gifu and *At* Col-0 were grown for 5 weeks in CAS soil (batch 15 from January 2020) in 7 × 7 cm pots alongside unplanted control pots under short-day conditions. Pots were watered with sterile water from the bottom as needed. Root, rhizosphere and soil samples were harvested and processed as described previously (Thiergart, T. et al., 2020). In total, 15, 13 and eight replicates were sampled for Col-0, Gifu and unplanted controls, respectively. DNA was isolated from those samples using the MP Biomedicals FastDNA Spin Kit for Soil. \ No newline at end of file