diff --git a/assays/MSA_dissection/dataset/LD_dissection_results.xlsx b/assays/MSA_dissection/dataset/LD_dissection_results.xlsx index 64dc4cf7dee6f3f8928601e992b509edbab94304..cef5b49514f1106dfd50693504a57d2a76025643 100644 Binary files a/assays/MSA_dissection/dataset/LD_dissection_results.xlsx and b/assays/MSA_dissection/dataset/LD_dissection_results.xlsx differ diff --git a/assays/MSA_dissection/dataset/SD_dissection_results.xlsx b/assays/MSA_dissection/dataset/SD_dissection_results.xlsx index c49bb87040186720fe0d2dacf60544887e0f70ed..14376c5e3c133d299b32af93300f368dbcdf5fab 100644 Binary files a/assays/MSA_dissection/dataset/SD_dissection_results.xlsx and b/assays/MSA_dissection/dataset/SD_dissection_results.xlsx differ diff --git a/assays/MSA_dissection/isa.assay.xlsx b/assays/MSA_dissection/isa.assay.xlsx index e499e2097c1670329209f5a36089f3ff5763fd1e..ae484969c38ff34f7bcb190cca69553f66397852 100644 Binary files a/assays/MSA_dissection/isa.assay.xlsx and b/assays/MSA_dissection/isa.assay.xlsx differ diff --git a/assays/plant_genotyping/dataset/lwd1_T0_genotype_results.xlsx b/assays/plant_genotyping/dataset/lwd1_T0_genotype_results.xlsx index 96afb610143e2363884ef5c7bf8c5049bc4b6ad9..10d155def6c987c0433a5303b6fd7d0aca186df0 100644 Binary files a/assays/plant_genotyping/dataset/lwd1_T0_genotype_results.xlsx and b/assays/plant_genotyping/dataset/lwd1_T0_genotype_results.xlsx differ diff --git a/assays/plant_genotyping/dataset/lwd1_T1_genotype_results.xlsx b/assays/plant_genotyping/dataset/lwd1_T1_genotype_results.xlsx index a67f4b724173653d2805843fb9797f5706c6b20b..7b871ed3a24b4e8ab0fa73fc7462b5536734994e 100644 Binary files a/assays/plant_genotyping/dataset/lwd1_T1_genotype_results.xlsx and b/assays/plant_genotyping/dataset/lwd1_T1_genotype_results.xlsx differ diff --git a/assays/plant_genotyping/isa.assay.xlsx b/assays/plant_genotyping/isa.assay.xlsx index a9d96125514603423bf9e6870a1d7aa11f20f89a..c8d4f3fe51b3b249880fcb1ae61f6976ac986dbc 100644 Binary files a/assays/plant_genotyping/isa.assay.xlsx and b/assays/plant_genotyping/isa.assay.xlsx differ diff --git a/assays/plant_propagation/isa.assay.xlsx b/assays/plant_propagation/isa.assay.xlsx index 5a72873bc8801f7cf9c7991d761c9dc296bbcfc2..8e27cc0f22538b2cc03b92bbaddc7d12e4088c68 100644 Binary files a/assays/plant_propagation/isa.assay.xlsx and b/assays/plant_propagation/isa.assay.xlsx differ diff --git a/assays/plant_propagation/protocols/plant_growth.md b/assays/plant_propagation/protocols/plant_growth.md new file mode 100644 index 0000000000000000000000000000000000000000..741380b06d61b291e9970da80677376ea7f7d849 --- /dev/null +++ b/assays/plant_propagation/protocols/plant_growth.md @@ -0,0 +1,10 @@ +## Growth of *lwd1* mutant plants + +- plants were grown until maturity + +**Growth conditions:** + +- long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR) +- 1.5 L pots (HerkuPlast Kubern GmbH, pot volume 75 cm^3) +- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) + diff --git a/assays/plant_propagation/protocols/plant_propagation.md b/assays/plant_propagation/protocols/plant_propagation.md new file mode 100644 index 0000000000000000000000000000000000000000..9796a061c289fc953518e4a6a6166fd9e07ef766 --- /dev/null +++ b/assays/plant_propagation/protocols/plant_propagation.md @@ -0,0 +1,4 @@ +## Propagation of *lwd1* mutant plants + +- plants were grown until full maturity +- seeds were harvested for each plant individually \ No newline at end of file diff --git a/assays/plant_propagation/protocols/progagation_of_mutant_plants.md b/assays/plant_propagation/protocols/progagation_of_mutant_plants.md deleted file mode 100644 index 3b3d59a549ec2d3ce2b3add9b9fa690095ed2c12..0000000000000000000000000000000000000000 --- a/assays/plant_propagation/protocols/progagation_of_mutant_plants.md +++ /dev/null @@ -1,8 +0,0 @@ -## Propagation of *lwd1* mutant plants - -- plants were grown in LD conditions until maturity -- seeds were harvested for each plant individually - -**Generations:** -- T0 plants: regenerated plants from cell culture (embryo transformation), produce T1 seeds -- T1 plants: genotyping for potential mutants, produce T2 seeds \ No newline at end of file diff --git a/studies/MSA_dissection_eam7/isa.study.xlsx b/studies/MSA_dissection_eam7/isa.study.xlsx index 1f5ffb3ff797d77f319dbcf87753ffe54682818c..75d5da03e0e41e557d4c8cf671e78174c373fb3c 100644 Binary files a/studies/MSA_dissection_eam7/isa.study.xlsx and b/studies/MSA_dissection_eam7/isa.study.xlsx differ diff --git a/studies/generating_lwd1_mutants/isa.study.xlsx b/studies/generating_lwd1_mutants/isa.study.xlsx index b73dc2b9b607e8f7aa8443b26ae46d7506742150..e5bf3cef8d3d72734f4cc39313d8dc9eba622d97 100644 Binary files a/studies/generating_lwd1_mutants/isa.study.xlsx and b/studies/generating_lwd1_mutants/isa.study.xlsx differ diff --git a/studies/phenotyping_eam7_plants/isa.study.xlsx b/studies/phenotyping_eam7_plants/isa.study.xlsx index 83b719ddecf9aa8711ff4f25c03315e8862453e8..f93130f983861ac646070573e9a56bae84895b6d 100644 Binary files a/studies/phenotyping_eam7_plants/isa.study.xlsx and b/studies/phenotyping_eam7_plants/isa.study.xlsx differ