diff --git a/assays/plant_genotyping/dataset/complementation_F1_genotype_results.xlsx b/assays/plant_genotyping/dataset/complementation_F1_genotype_results.xlsx
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diff --git a/assays/plant_genotyping/protocols/complementation_plant_genotyping.md b/assays/plant_genotyping/protocols/complementation_plant_genotyping.md
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+**F1 plants of lwd/eam7 allelism test: genoypting **
+
+- DNA was extracted from leaves of all plants using QIAGEN_DNeasy-Plant_mini protocol
+- plants were tested for possible mutations in the CDS of LWD1 by amplifying the full genomic sequence using the primers LWD1_71us_F (5'-ATCAGACCCCTCCAACGACT-3') and LWD1_21ds_R (5'-TTCCTCTTTCTACCCCACCA-3')
+- the PCR fragments were Sanger sequenced using two different primers:
+	- LWD1_71us_F (test the beginning of the CDS)
+	- LWD1_qPCR114F (5'-CAGCTCATAGCCCATGACAA-3') (test the end of the CDS)
+- all mutations and the zygosity of the mutations were determined relative to the start codon of LWD1
+
+- all F1 plants were genotyped
+- only some parent plants were genotyped again
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