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lwd/eam7 allelism test: genoypting ** + +- DNA was extracted from leaves of all plants using QIAGEN_DNeasy-Plant_mini protocol +- plants were tested for possible mutations in the CDS of LWD1 by amplifying the full genomic sequence using the primers LWD1_71us_F (5'-ATCAGACCCCTCCAACGACT-3') and LWD1_21ds_R (5'-TTCCTCTTTCTACCCCACCA-3') +- the PCR fragments were Sanger sequenced using two different primers: + - LWD1_71us_F (test the beginning of the CDS) + - LWD1_qPCR114F (5'-CAGCTCATAGCCCATGACAA-3') (test the end of the CDS) +- all mutations and the zygosity of the mutations were determined relative to the start codon of LWD1 + +- all F1 plants were genotyped +- only some parent plants were genotyped again \ No newline at end of file -- GitLab