From 159481e56c5d6b6a3fcf8ebb0bdae4b0825fb80d Mon Sep 17 00:00:00 2001 From: Gesa Helmsorig <gesa.helmsorig@hhu.de> Date: Wed, 13 Nov 2024 08:15:40 +0100 Subject: [PATCH] edited growth conditions + readme --- studies/allelism_test/README.md | 19 +++++++++++++++++++ .../plant_material_growth_conditions.md | 2 +- 2 files changed, 20 insertions(+), 1 deletion(-) diff --git a/studies/allelism_test/README.md b/studies/allelism_test/README.md index e69de29..65705fe 100644 --- a/studies/allelism_test/README.md +++ b/studies/allelism_test/README.md @@ -0,0 +1,19 @@ +**Allelism test** + +Complementation crosses were performed to confirm that *lwd1* and *eam7* are allelic, which are both recessive mutations. For this, homozygous *lwd1* mutants *lwd1-26*, *lwd1-390*, and *lwd1-402* were crossed with BW(Ppd-H1, eam7). F1 plants were grown under SD conditions and flowering time was scored. If *lwd1* and *eam7* are allelic, we expected the F1 plants to display the mutant phenotype and be early flowering under SD. + +**study overview** + + parent plants (lwd1 mutants, BW(Ppd-H1,eam7), BW(Ppd-H1), GP-fast) + + -> assay: complementation_crosses + output: F1 plants + + -> assay: plant_genotyping + output: F1 genotype information + + -> assay: plant_phenotyping + output: F1 flowering data + + +Results can be found in Figure 6 and Supplemental Figure S8. \ No newline at end of file diff --git a/studies/allelism_test/protocols/plant_material_growth_conditions.md b/studies/allelism_test/protocols/plant_material_growth_conditions.md index 531bc37..dcb4957 100644 --- a/studies/allelism_test/protocols/plant_material_growth_conditions.md +++ b/studies/allelism_test/protocols/plant_material_growth_conditions.md @@ -32,4 +32,4 @@ parents: Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110. 166249 -Gol L, Haraldsson EB, von Korff M. Ppd-H1 integrates drought stress signals to control spike development and flowering time in barley. J Exp Bot. 2021:72(1):122–136. https://doi.org/10.1093/jxb/eraa261 +Gol L, Haraldsson EB, von Korff M. Ppd-H1 integrates drought stress signals to control spike development and flowering time in barley. J Exp Bot. 2021:72(1):122–136. https://doi.org/10.1093/jxb/eraa261 \ No newline at end of file -- GitLab