From 1e8a0e3cce68d1dfccbe54373a1463410e0c41ba Mon Sep 17 00:00:00 2001 From: Gesa Helmsorig <gesa.helmsorig@hhu.de> Date: Thu, 14 Nov 2024 10:34:31 +0000 Subject: [PATCH] Update README.md --- .../README.md | 24 +++++++------------ 1 file changed, 9 insertions(+), 15 deletions(-) diff --git a/studies/gene_expression_lwd1_mutants_diurnal/README.md b/studies/gene_expression_lwd1_mutants_diurnal/README.md index a3cfcbf..8609007 100644 --- a/studies/gene_expression_lwd1_mutants_diurnal/README.md +++ b/studies/gene_expression_lwd1_mutants_diurnal/README.md @@ -2,24 +2,18 @@ 2-week old plants from the genotypes *lwd1-26*, *lwd1-390*, *lwd1-402*, and GP-fast were sampled at Zeitgeber Time (ZT) 0, 4, 8, 12, 16, and 20. -Gene expression of CO1, ELF3, FT1, LUX1, LWD1, PHYB, PHYC, Ppd-H1, and PRR1 were tested. +Gene expression of *CO1, ELF3, FT1, LUX1, LWD1, PHYB, PHYC, Ppd-H1*, and *PRR1* were tested. **Study overview:** - input: 2-weeks old lwd1-26, lwd1-390, lwd1-402 and GP-fast plants grown under SD. - - -> assay: leaf_sampling - output: frozen leaf tissue (3 biol. replicates) - - -> assay: RNA_extraction - output: RNA samples - - -> assay: cDNA_synthesis - output: cDNA samples - - -> assay: qRT-PCR - output: gene expression data (2 techn. replicates) - +```mermaid + flowchart TD; + A[lwd1 mutants and GP-fast] --> |leaf_sampling| B[frozen leaf tissues, 3 biol. replicates] + B --> |RNA_extraction| C[RNA samples] + C --> |cDNA_synthesis| D[cDNA samples] + D --> |qRT-PCR| E[gene expression data, 2 techn. replicates] +``` +**Publication:** Results can be found in Figure 5D-L. \ No newline at end of file -- GitLab