diff --git a/assays/DNA_extraction/isa.assay.xlsx b/assays/DNA_extraction/isa.assay.xlsx index 7c73d7b6112fad69cc08d4f3835e93548bae52f9..1d87c5dd264ac21e8b7a2c363bd1ace1a8d995c2 100644 Binary files a/assays/DNA_extraction/isa.assay.xlsx and b/assays/DNA_extraction/isa.assay.xlsx differ diff --git a/assays/complementation_crosses/isa.assay.xlsx b/assays/complementation_crosses/isa.assay.xlsx index d178c9235902fc95ba80cbeac24db9515227c7c5..fe826661a6d9f2e78a6e4e33fc6a92f45c60beda 100644 Binary files a/assays/complementation_crosses/isa.assay.xlsx and b/assays/complementation_crosses/isa.assay.xlsx differ diff --git a/assays/complementation_crosses/protocols/crossing_plants.md b/assays/complementation_crosses/protocols/crossing_plants.md index 59222e7927c797078be387fcfa9d618554f9e6b8..4d27063790916eb10783c1610dbb5b2ddebdc015 100644 --- a/assays/complementation_crosses/protocols/crossing_plants.md +++ b/assays/complementation_crosses/protocols/crossing_plants.md @@ -7,5 +7,5 @@ **Crossing:** - emasculate florets from mother plants before pollen are shed (about Waddington stage 8.5 - 9.0) - pollinate florets with pollen from father plants, pollinate all florets from one spike with the same father: this is one cross -- cover the spike with a paper bag, count later how many of the pollinated florets develop into seeds +- cover the spike with a paper bag - harvest seeds from each spike / each cross individually when they are completely dry \ No newline at end of file diff --git a/assays/complementation_crosses/protocols/score_crosses.md b/assays/complementation_crosses/protocols/score_crosses.md new file mode 100644 index 0000000000000000000000000000000000000000..4cdd2969d924a5a9bb5839f9f01dae117f040265 --- /dev/null +++ b/assays/complementation_crosses/protocols/score_crosses.md @@ -0,0 +1,5 @@ +## Score successful crosses + +- all crossed spikes were covered with a paper bag +- number of pollinated florets per spike were documented +- when seeds were ripe: count how many of the polinated florets developed into seeds \ No newline at end of file diff --git a/assays/plant_genotyping/dataset/complementation_F1_genotype_results.xlsx b/assays/plant_genotyping/dataset/complementation_F1_genotype_results.xlsx index 5f9124b7246df4bb4094ddf60a5c890768005d5a..46c21e23a7e53b3bd6509d96757a627bf0847fa5 100644 Binary files a/assays/plant_genotyping/dataset/complementation_F1_genotype_results.xlsx and b/assays/plant_genotyping/dataset/complementation_F1_genotype_results.xlsx differ diff --git a/assays/plant_genotyping/isa.assay.xlsx b/assays/plant_genotyping/isa.assay.xlsx index c8d4f3fe51b3b249880fcb1ae61f6976ac986dbc..a86ad8981cbc876662ca928aad5f1e8443b278e7 100644 Binary files a/assays/plant_genotyping/isa.assay.xlsx and b/assays/plant_genotyping/isa.assay.xlsx differ diff --git a/assays/plant_phenotyping/dataset/complementation_F1_phenotyping_results.xlsx b/assays/plant_phenotyping/dataset/complementation_F1_phenotyping_results.xlsx index a414e5143d7f6ef0abac3ce565dbcec45ccdc47e..3a4c21624884d094742c494279271b4af82b50d5 100644 Binary files a/assays/plant_phenotyping/dataset/complementation_F1_phenotyping_results.xlsx and b/assays/plant_phenotyping/dataset/complementation_F1_phenotyping_results.xlsx differ diff --git a/assays/plant_phenotyping/isa.assay.xlsx b/assays/plant_phenotyping/isa.assay.xlsx index 88492435eb548bedb2550be238457f93f530e114..ec6fbad26cd705929999f131b4c66d0aee51a7c7 100644 Binary files a/assays/plant_phenotyping/isa.assay.xlsx and b/assays/plant_phenotyping/isa.assay.xlsx differ diff --git a/studies/allelism_test/isa.study.xlsx b/studies/allelism_test/isa.study.xlsx index d2788a764bd50f332e57d36776e95dfa975b195f..1f3b6b71f9d7abc96a1a60f8ae72a11132baba7d 100644 Binary files a/studies/allelism_test/isa.study.xlsx and b/studies/allelism_test/isa.study.xlsx differ