diff --git a/assays/biparental_mapping/dataset/introgression_area.xlsx b/assays/biparental_mapping/dataset/introgression_area.xlsx
index 27aae1684bdcebb48999103f0973eaf1ee3c02d4..62c8a756ef535a3ab9b15229132abebded799093 100644
Binary files a/assays/biparental_mapping/dataset/introgression_area.xlsx and b/assays/biparental_mapping/dataset/introgression_area.xlsx differ
diff --git a/assays/biparental_mapping/dataset/target_genes_results.md b/assays/biparental_mapping/dataset/target_genes_results.md
new file mode 100644
index 0000000000000000000000000000000000000000..838ca4e835050546ec6dc135897a481a94f37dc0
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+++ b/assays/biparental_mapping/dataset/target_genes_results.md
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+**related to the circadian clock**
+
+MorexV3 gene ID	pident	evalue	Araport11 gene ID	Gene name	SNP positions	Changes in aa sequence
+HORVU.MOREX.r3.6HG0592840	73.75	2.26E-40	AT2G21660	GR-RBP1	None	None
+HORVU.MOREX.r3.6HG0583670	69.309	0	AT1G12910	LWD1	32 bp del (1219-1250)	A407F
+						N408S
+						K409F
+						L410*
+HORVU.MOREX.r3.6HG0587680	66.082	0	AT4G08920	CRY1	None	None
+HORVU.MOREX.r3.6HG0595250	38.475	3.92E-106	AT5G61380	PRR1	A642G T1300C	T215A
+						S434P
+HORVU.MOREX.r3.6HG0578070	55.367	0	AT5G51200	EDS4	-	-
+HORVU.MOREX.r3.6HG0589220	34.855	9.66E-46	AT3G07640	PBAC5	-	-
+HORVU.MOREX.r3.6HG0571470	36.735	1.3	AT3G42170	DAY-SLEEPER	-	-
+HORVU.MOREX.r3.6HG0582650	30.108	1.00E-12	AT3G42170	DAY-SLEEPER	-	-
+HORVU.MOREX.r3.6HG0592100	29.042	1.69E-45	AT3G42170	DAY-SLEEPER	-	-
+HORVU.MOREX.r3.6HG0571480	28.319	1.11E-13	AT3G42170	DAY-SLEEPER	-	-
+
+
+**associated with early flowering**
+
+PSEUDO RESPONSE REGULATOR 1 (PRR1)				HORVU.MOREX.r3.6HG0595250
+CRYPTOCHROME 1a (CRY1a)							HORVU.MOREX.r3.6HG0587680)
+GLYCIN-RICH RNA-BINDING-PROTEIN 1 (GR-RBP1)		HORVU.MOREX.r3.6HG0592840)
+LIGHT-REGULATED WD 1 (LWD1)						HORVU.MOREX.r3.6HG0583670
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diff --git a/assays/biparental_mapping/protocols/target_gene_identification.txt b/assays/biparental_mapping/protocols/target_gene_identification.txt
new file mode 100644
index 0000000000000000000000000000000000000000..35443c2f269a7df6ad32b809a28ff3525f878d71
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+
+- based on the CAPS marker results, the introgression area could be reduced to 2.98 cM/286 Mbp (Morex V3, Mascher et al. 2021)
+
+flanking markers: 
+JHI-Hv50k-2016-390119	96639872 bp
+SCRI_RS_114351			382834297 bp
+
+
+- this region contains 1084 high confidence (HC) genes
+- Arabidopsis (Araport11, Cheng et al. 2017) homologs were indentified via BLASTP
+- 10 of the genes genes were homologous to Arabidopsis genes with functions in the circadian clock -> target_genes
+- 4 of these genes were associated with early flowering in barley or Arabidopsis -> target genes
+- 1 had a consistent mutation in eam7 vs EAM7 plants
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