diff --git a/assays/plant_propagation/protocols/progagation_of_mutant_plants.md b/assays/plant_propagation/protocols/progagation_of_mutant_plants.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..3b3d59a549ec2d3ce2b3add9b9fa690095ed2c12 100644
--- a/assays/plant_propagation/protocols/progagation_of_mutant_plants.md
+++ b/assays/plant_propagation/protocols/progagation_of_mutant_plants.md
@@ -0,0 +1,8 @@
+## Propagation of *lwd1* mutant plants
+
+- plants were grown in LD conditions until maturity
+- seeds were harvested for each plant individually
+
+**Generations:**
+- T0 plants: regenerated plants from cell culture (embryo transformation), produce T1 seeds
+- T1 plants: genotyping for potential mutants, produce T2 seeds
\ No newline at end of file