From 2515ba3b6afcbd078ff49d44b914132ce4a83606 Mon Sep 17 00:00:00 2001
From: Gesa Helmsorig <gesa.helmsorig@hhu.de>
Date: Wed, 13 Nov 2024 13:58:38 +0000
Subject: [PATCH] Update file progagation_of_mutant_plants.md

---
 .../protocols/progagation_of_mutant_plants.md             | 8 ++++++++
 1 file changed, 8 insertions(+)

diff --git a/assays/plant_propagation/protocols/progagation_of_mutant_plants.md b/assays/plant_propagation/protocols/progagation_of_mutant_plants.md
index e69de29..3b3d59a 100644
--- a/assays/plant_propagation/protocols/progagation_of_mutant_plants.md
+++ b/assays/plant_propagation/protocols/progagation_of_mutant_plants.md
@@ -0,0 +1,8 @@
+## Propagation of *lwd1* mutant plants
+
+- plants were grown in LD conditions until maturity
+- seeds were harvested for each plant individually
+
+**Generations:**
+- T0 plants: regenerated plants from cell culture (embryo transformation), produce T1 seeds
+- T1 plants: genotyping for potential mutants, produce T2 seeds
\ No newline at end of file
-- 
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