From 254ce7b5ece332fa0f602a0950ffb074189adb1f Mon Sep 17 00:00:00 2001
From: Gesa Helmsorig <gesa.helmsorig@hhu.de>
Date: Wed, 13 Nov 2024 12:22:17 +0000
Subject: [PATCH] Update file diurnal_plant_material_growth_conditions.md

---
 ...iurnal_plant_material_growth_conditions.md | 39 ++++++++++---------
 1 file changed, 20 insertions(+), 19 deletions(-)

diff --git a/studies/gene_expression_eam7_plants_diurnal/protocols/diurnal_plant_material_growth_conditions.md b/studies/gene_expression_eam7_plants_diurnal/protocols/diurnal_plant_material_growth_conditions.md
index 6e3d889..35f54b3 100644
--- a/studies/gene_expression_eam7_plants_diurnal/protocols/diurnal_plant_material_growth_conditions.md
+++ b/studies/gene_expression_eam7_plants_diurnal/protocols/diurnal_plant_material_growth_conditions.md
@@ -1,9 +1,10 @@
-**eam7 diurnal expression: plant material and growth conditions**
+## *eam7* diurnal expression: plant material and growth conditions
 
-**plant material*
+**Plant material:**
 
-species: Hordeum vulgare
-genotypes:
+Species: *Hordeum vulgare*
+
+Genotypes:
 
 - BW:  Bowman, spring cultivar
 - BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011)
@@ -11,33 +12,33 @@ genotypes:
 - BW(Ppd-H1, eam7): cross of the two introgression lines
 
 
-**growth condtions**
+**Growth condtions:**
 
 - short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
 - QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3)
 - Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
 
 
-**sampling**
+**Sampling:**
 
 - 2 weeks old plants
 - youngest, fully elongated leaf: leaf 2
 - sample every 2 hours over 24 hours: 
-	ZT0 (lights are turned on)
-	ZT2
-	ZT4
-	ZT6
-	ZT8 (lights are turned off)
-	ZT10
-	ZT12
-	ZT14
-	ZT16
-	ZT18
-	ZT20
-	ZT22
+	- ZT0 (lights are turned on)
+	- ZT2
+	- ZT4
+	- ZT6
+	- ZT8 (lights are turned off)
+	- ZT10
+	- ZT12
+	- ZT14
+	- ZT16
+	- ZT18
+	- ZT20
+	- ZT22
 	
 	
-**References**
+**References:**
 
 Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.166249
 
-- 
GitLab