From 32cfeca091e55f4c16ab8ac74615a8f7d5aaa933 Mon Sep 17 00:00:00 2001
From: Gesa Helmsorig <gesa.helmsorig@hhu.de>
Date: Tue, 5 Nov 2024 12:28:12 +0100
Subject: [PATCH] added protocol

---
 .../protocols/crossing_plants.md                       | 10 ++++++++++
 1 file changed, 10 insertions(+)

diff --git a/assays/complementation_crosses/protocols/crossing_plants.md b/assays/complementation_crosses/protocols/crossing_plants.md
index e69de29..b090a5e 100644
--- a/assays/complementation_crosses/protocols/crossing_plants.md
+++ b/assays/complementation_crosses/protocols/crossing_plants.md
@@ -0,0 +1,10 @@
+**crossing of plants**
+
+- grow parent plants in LD conditions
+- if parental plants have very different flowering times: sow the faster one with a bit of delay)
+
+
+- emasculate florets from mother plants before pollen are shed (about Waddington stage 8.5 - 9.0)
+- pollinate florets with pollen from father plants, pollinate all florets from one spike with the same father: this is one cross
+- cover the spike with a paper bag, count later how many of the pollinated florets develop into seeds
+- harvest seeds from each spike / each cross individually when they are completely dry
\ No newline at end of file
-- 
GitLab