From 3380b70d4a11ebb2f03af4969ca61a5c16f6bc07 Mon Sep 17 00:00:00 2001 From: Gesa Helmsorig <gesa.helmsorig@hhu.de> Date: Wed, 13 Nov 2024 12:31:45 +0000 Subject: [PATCH] Update 2 files - /studies/phenotyping_lwd1_mutants/protocols/plant_material_growth_conditions.md - /studies/phenotyping_lwd1_mutants/README.md --- studies/phenotyping_lwd1_mutants/README.md | 9 +++++---- .../protocols/plant_material_growth_conditions.md | 13 +++++++------ 2 files changed, 12 insertions(+), 10 deletions(-) diff --git a/studies/phenotyping_lwd1_mutants/README.md b/studies/phenotyping_lwd1_mutants/README.md index 195ea39..535a546 100644 --- a/studies/phenotyping_lwd1_mutants/README.md +++ b/studies/phenotyping_lwd1_mutants/README.md @@ -1,16 +1,17 @@ -**Phenotype *lwd1* mutants under SD conditions** +## Phenotype *lwd1* mutants under SD conditions Grow *lwd* mutants and the wild-type GP-fast under short-day (SD) conditions. Score flowering time and phenotypic traits at flowering and at harvest when plants are fully dry. -**study overview** +**Study overview:** -input: lwd1-26, lwd1-390, lwd1-402, GP-fast (WT) + input: lwd1-26, lwd1-390, lwd1-402, GP-fast (WT) -> assay: plant_phenotyping output: - flowering time (in DAE) - at flowering: plant height, leaf number, tiller number - - at harvest (on main culm): spike length, floret and grain number, spike fertility + - at harvest (on main culm): spike length, floret and grain number, + spike fertility Results can be found in Figure 5A-C and Supplemental Figure S7. \ No newline at end of file diff --git a/studies/phenotyping_lwd1_mutants/protocols/plant_material_growth_conditions.md b/studies/phenotyping_lwd1_mutants/protocols/plant_material_growth_conditions.md index 659381a..bf18274 100644 --- a/studies/phenotyping_lwd1_mutants/protocols/plant_material_growth_conditions.md +++ b/studies/phenotyping_lwd1_mutants/protocols/plant_material_growth_conditions.md @@ -1,22 +1,23 @@ -**Plant material and growth conditions** +## Plant material and growth conditions -**plant material* +**Plant material:** -species: Hordeum vulgare -genotypes: +Species: *Hordeum vulgare* + +Genotypes: - GP-fast: WT, Ppd-H1 introgression line in Golden Promise background, lwd1 mutant background, Gol et al. (2021) - lwd1-26: lwd1 mutant in GP-fast background, truncated protein of 26 aa - lwd1-390: lwd1 mutant in GP-fast background, truncated protein of 390 aa - lwd1-402: lwd1 mutant in GP-fast background, truncated protein of 402 aa -**growth condtions** +**Growth condtions:** - short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3) - Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) -**References** +**References:** Gol L, Haraldsson EB, von Korff M. Ppd-H1 integrates drought stress signals to control spike development and flowering time in barley. J Exp Bot. 2021:72(1):122–136. https://doi.org/10.1093/jxb/eraa261 \ No newline at end of file -- GitLab