From 39390f3927c415df12eba7913578720890b85872 Mon Sep 17 00:00:00 2001
From: Gesa Helmsorig <gesa.helmsorig@hhu.de>
Date: Wed, 13 Nov 2024 12:57:50 +0000
Subject: [PATCH] Update file lwd1_plant_genotyping.md

---
 assays/plant_genotyping/protocols/lwd1_plant_genotyping.md | 6 ++++--
 1 file changed, 4 insertions(+), 2 deletions(-)

diff --git a/assays/plant_genotyping/protocols/lwd1_plant_genotyping.md b/assays/plant_genotyping/protocols/lwd1_plant_genotyping.md
index 0a4205e..be2bdbe 100644
--- a/assays/plant_genotyping/protocols/lwd1_plant_genotyping.md
+++ b/assays/plant_genotyping/protocols/lwd1_plant_genotyping.md
@@ -1,4 +1,6 @@
-**lwd1 T0 and T1 plants **
+## Genotyping of *lwd1* mutants
+
+***lwd1* T0 and T1 plants:**
 
 - DNA was extracted from leaves of all mutants using QIAGEN_DNeasy-Plant_mini protocol
 - plants were tested for possible mutations in the CDS of LWD1 by amplifying the full genomic sequence using the primers LWD1_71us_F (5'-ATCAGACCCCTCCAACGACT-3') and LWD1_21ds_R (5'-TTCCTCTTTCTACCCCACCA-3')
@@ -7,5 +9,5 @@
 	- plants transformed with pMGE599/182-1222 (approach 2): LWD1_qPCR114F (5'-CAGCTCATAGCCCATGACAA-3')
 - mutations and the zygosity of the mutations were determined relative to the start codon of LWD1
 
-**only in T1 plants**:
+**Only in T1 plants:**:
 - PCR was performed on transformation vector to check presence/absence of Cas9 using the primers pMGE599_M13F (5'-GTTTTCCCAGTCACGAC-3') and pMGE599_R (5'-TGTCGACATCGATAAGCTTGAA-3') 
\ No newline at end of file
-- 
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