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diff --git a/assays/leaf_sampling/README.md b/assays/leaf_sampling/README.md
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diff --git a/assays/leaf_sampling/protocols/leaf_sampling.txt b/assays/leaf_sampling/protocols/leaf_sampling.txt
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+leaf sampling for RNA extraction
+
+-	place two 3 mm metal beads into 2.0 ml tube
+-	always sample the youngest, fully elongated leaf (auricles are clearly visible)
+-	sample three biological replicates for each genotype and time point
+-	for each biological replicate: pool the leaf from two plants into one tube
+-	freeze samples directly in liquid nitrogen
+-	Store samples at -80°C until needed
\ No newline at end of file
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