diff --git a/assays/qRT-PCR/dataset/eam7_development_gene_expression.xlsx b/assays/qRT-PCR/dataset/eam7_development_gene_expression.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..e5a2b35cc1ca83069f9edbf8d69201279eb25be8 Binary files /dev/null and b/assays/qRT-PCR/dataset/eam7_development_gene_expression.xlsx differ diff --git a/assays/qRT-PCR/dataset/eam7_diurnal_gene_expression.xlsx b/assays/qRT-PCR/dataset/eam7_diurnal_gene_expression.xlsx index 86d4e04bc9698abcf0e923fbfbf427ceeeef6920..571943e8fee0ca147604041227db0c04212b4516 100644 Binary files a/assays/qRT-PCR/dataset/eam7_diurnal_gene_expression.xlsx and b/assays/qRT-PCR/dataset/eam7_diurnal_gene_expression.xlsx differ diff --git a/assays/qRT-PCR/isa.assay.xlsx b/assays/qRT-PCR/isa.assay.xlsx index 882b17594832763d8329127e9a9fff7897582348..b36329e99be1f3e8e294ee530d9b0dadbdae3e38 100644 Binary files a/assays/qRT-PCR/isa.assay.xlsx and b/assays/qRT-PCR/isa.assay.xlsx differ diff --git a/assays/qRT-PCR/protocols/qRT-PCR_primer.txt b/assays/qRT-PCR/protocols/qRT-PCR_primer.txt index f85e56b80c5dc6282ea7b94bb4e368a689c12d11..142186ea98a706bccc08b68d4b5635f41ccf4dc8 100644 --- a/assays/qRT-PCR/protocols/qRT-PCR_primer.txt +++ b/assays/qRT-PCR/protocols/qRT-PCR_primer.txt @@ -1,35 +1,41 @@ -Target gene Primer Sequence 5’-3’ Fragment size (bp) -ACT (HORVU.MOREX.r3.5HG0457850) ACT_591F CGTGTTGGATTCTGGTGATG 208 - ACT_789R AGCCACATATGCGAGCTTCT -CO1 (HORVU.MOREX.r3.7HG0671540) CO1_2185F CTGCTGGGGCTAGTGCTTAC 251 - CO1_3454R CCTTGTTGCATAACGTGTGG -CO2 (HORVU.MOREX.r3.6HG0611630) CO2_564F AGTGGACTCTTGGCTCCTCA 158 - CO2_721R CATGCTGCTGTTCTTGCATT -ELF3 (HORVU.MOREX.r3.1HG0095050) ELF3_DL_3060_F3 TGCTGTCCAAGTGTTTGAGC 242 - Elf3_DL_4483_R3 CCTGGTTTCCTTCGGTGTTA -FT1 (HORVU.MOREX.r3.7HG0653910) FT1_1955F GGTAGACCCAGATGCTCCAA 120 - FT1_2183R TCGTAGCACATCACCTCCTG -GI (HORVU.MOREX.r3.3HG0238250) GI_6780F TCAGTTAGAGCTCCTGGAAGT 263 - GI_7289R GGTAGTTTGGGCTTTGGATG -LHY (HORVU.MOREX.r3.7HG0699010) HvLHY_672F CCTGGAATTGGAGATGGAGA 210 - HvLHY_882R TGAGCATGGCTTCTGATTTG -LUX1 (HORVU.MOREX.r3.3HG0328340) LUX_1077F AATTCAGTCCACGGATGCTC 222 - LUX_1298R CTTCACTTCAGCTCCCCTTG -LWD1 (HORVU.MOREX.r3.6HG0583670) LWD1_qPCR-114_F CAGCTCATAGCCCATGACAA 114 +Target gene Primer Sequence 5’-3’ Fragment size (bp) +ACT (HORVU.MOREX.r3.5HG0457850) ACT_591F CGTGTTGGATTCTGGTGATG 208 + ACT_789R AGCCACATATGCGAGCTTCT +CO1 (HORVU.MOREX.r3.7HG0671540) CO1_2185F CTGCTGGGGCTAGTGCTTAC 251 + CO1_3454R CCTTGTTGCATAACGTGTGG +CO2 (HORVU.MOREX.r3.6HG0611630) CO2_564F AGTGGACTCTTGGCTCCTCA 158 + CO2_721R CATGCTGCTGTTCTTGCATT +ELF3 (HORVU.MOREX.r3.1HG0095050) ELF3_DL_3060_F3 TGCTGTCCAAGTGTTTGAGC 242 + Elf3_DL_4483_R3 CCTGGTTTCCTTCGGTGTTA +FT1 (HORVU.MOREX.r3.7HG0653910) FT1_1955F GGTAGACCCAGATGCTCCAA 120 + FT1_2183R TCGTAGCACATCACCTCCTG +FT2 (HORVU.MOREX.r3.3HG0244930 FT2_4031R TCACATCCTTCTCCCGCCGG 238 + FT2_3809_F TACCGAGGTTGTGTGCTACG +FT3 (HORVU.MOREX.r3.1HG0077250) FT3-1004F TGTCAGACATCCCTGGAACA 225 + FT3-1848R CTGATCCACCTTCCCTTTGA +FT4 (HORVU.MOREX.r3.2HG0117260) FT4_626F AAACGATAGCTTTGGAAAAGAGATC 230 + FT4_2537R TCCTCCCGCCGGTGCCCGTG +GI (HORVU.MOREX.r3.3HG0238250) GI_6780F TCAGTTAGAGCTCCTGGAAGT 263 + GI_7289R GGTAGTTTGGGCTTTGGATG +LHY (HORVU.MOREX.r3.7HG0699010) HvLHY_672F CCTGGAATTGGAGATGGAGA 210 + HvLHY_882R TGAGCATGGCTTCTGATTTG +LUX1 (HORVU.MOREX.r3.3HG0328340) LUX_1077F AATTCAGTCCACGGATGCTC 222 + LUX_1298R CTTCACTTCAGCTCCCCTTG +LWD1 (HORVU.MOREX.r3.6HG0583670) LWD1_qPCR-114_F CAGCTCATAGCCCATGACAA 114 LWD1_qPCR-114_R CTCCTTGTCCCGAAGATCAA -PHYB (HORVU.MOREX.r3.4HG0381880) HvPHYB-1151F CTTGCGCACCAACTATCAGA 232 - HvPHYB-1383R CTCCATGACACACCGTCAAC -PHYC (HORVU.MOREX.r3.5HG0511090) PHYC_0986F ACTACCCGGCAACTGACATC 142 - PHYC_1127R GAGCCACAGAGGCTGATAGG -Ppd-H1 (HORVU.MOREX.r3.2HG0107710) PPD1_2165F GATGGATTCAAAGGCAAGGA 172 - PPD1_2336R GAACAATTGGCTCCTCCAAA -PRR1 (HORVU.MOREX.r3.6HG0595250) HvPRR1_1056F GAGCATAGCATGGCACTTCA 237 - HvPRR1_1292R TGTCTTTCCTCGGAAATTGG -PRR59 (HORVU.MOREX.r3.4HG0350690) PRR59_2064_F GAAATTCCGCATGAAAAGGA 148 - PRR59_2212_R TTCCGCATCTTCTGTTGTTG -PRR73 (HORVU.MOREX.r3.5HG0498830) PRR73_441F GCGCCGTAGAGAATCAGAAC 222 - PRR73_662R CATGTCGGGTACAGTCATCG -PRR95 (HORVU.MOREX.r3.5HG0498830) PRR95_1467F CAGAACTCCAGTGTCGCAAA 251 +PHYB (HORVU.MOREX.r3.4HG0381880) HvPHYB-1151F CTTGCGCACCAACTATCAGA 232 + HvPHYB-1383R CTCCATGACACACCGTCAAC +PHYC (HORVU.MOREX.r3.5HG0511090) PHYC_0986F ACTACCCGGCAACTGACATC 142 + PHYC_1127R GAGCCACAGAGGCTGATAGG +Ppd-H1 (HORVU.MOREX.r3.2HG0107710) PPD1_2165F GATGGATTCAAAGGCAAGGA 172 + PPD1_2336R GAACAATTGGCTCCTCCAAA +PRR1 (HORVU.MOREX.r3.6HG0595250) HvPRR1_1056F GAGCATAGCATGGCACTTCA 237 + HvPRR1_1292R TGTCTTTCCTCGGAAATTGG +PRR59 (HORVU.MOREX.r3.4HG0350690) PRR59_2064_F GAAATTCCGCATGAAAAGGA 148 + PRR59_2212_R TTCCGCATCTTCTGTTGTTG +PRR73 (HORVU.MOREX.r3.5HG0498830) PRR73_441F GCGCCGTAGAGAATCAGAAC 222 + PRR73_662R CATGTCGGGTACAGTCATCG +PRR95 (HORVU.MOREX.r3.5HG0498830) PRR95_1467F CAGAACTCCAGTGTCGCAAA 251 PRR95_1717R TGCTGTTGCCAGAGTTGTTC -VRN1 (HORVU.MOREX.r3.5HG0511210) HVBM5a_292F CTGAAGGCGAAGGTTGAGAC 203 +VRN1 (HORVU.MOREX.r3.5HG0511210) HVBM5a_292F CTGAAGGCGAAGGTTGAGAC 203 HVBM5a_494R TTCTCCTCCTGCAGTGACCT \ No newline at end of file