diff --git a/studies/generating_lwd1_mutants/README.md b/studies/generating_lwd1_mutants/README.md index 78cbeced522dcb6ae9afc8c5b553ae57c9bf5e5b..2cb4fbb6af8359e4d4dfdf013f5f5289784a04ca 100644 --- a/studies/generating_lwd1_mutants/README.md +++ b/studies/generating_lwd1_mutants/README.md @@ -1,3 +1,5 @@ +Using CRISPR-Cas9 to generate *lwd1* mutants to confirm *LWD1* as the gene underlying the *eam7* locus. GP-fast embryos were transformed with vectors carrying sgRNAs that target the start or the end of the *LWD1* coding sequence. + **study overview** WT transformation plants (GP-fast) diff --git a/studies/genotyping_lwd1_mutants/README.md b/studies/genotyping_lwd1_mutants/README.md index dd767b8413438ac3dd2f0c57644eed9dd3c72311..5d85a7607d824ac216c915df00eb230eb079f9d4 100644 --- a/studies/genotyping_lwd1_mutants/README.md +++ b/studies/genotyping_lwd1_mutants/README.md @@ -1,11 +1,17 @@ -*study overview* +Using CRISPR-Cas9 to generate *lwd1* mutants to confirm LWD1 as the gene underlying the *eam7* locus. Mutants were genotyped by Sanger sequencing. + +**study overview** -T0 mutant plants (from study "generating_lwd1_mutants" --> assay: mutant_genotyping - output: T0 mutant genotype info --> assay: plant propagation - output: T1 mutant seeds - -> assay: mutant_genotyping - output: T1 mutant genotype info + T0 mutant plants (from study "generating_lwd1_mutants" + + -> assay: mutant_genotyping + output: T0 mutant genotype info + + -> assay: plant propagation + output: T1 mutant seeds + + -> assay: plant_genotyping + output: T1 mutant genotype info + -> assay: plant propagation - output: T2 mutant seeds (used in studies "gene_expression_lwd1_mutants" and "phenotyping_lwd1_mutants) \ No newline at end of file + output: T2 mutant seeds (used in studies gene_expression_lwd1_mutants" and "phenotyping_lwd1_mutants) \ No newline at end of file