From 62287324b687dbbc261ea00dc5436ce2e54d1361 Mon Sep 17 00:00:00 2001 From: Gesa Helmsorig <gesa.helmsorig@hhu.de> Date: Wed, 23 Oct 2024 12:40:06 +0200 Subject: [PATCH] changed protocol --- .../protocols/CAPS_marker_analysis.txt | 14 +++++++------- 1 file changed, 7 insertions(+), 7 deletions(-) diff --git a/assays/biparental_mapping/protocols/CAPS_marker_analysis.txt b/assays/biparental_mapping/protocols/CAPS_marker_analysis.txt index 292dc39..9b7d390 100644 --- a/assays/biparental_mapping/protocols/CAPS_marker_analysis.txt +++ b/assays/biparental_mapping/protocols/CAPS_marker_analysis.txt @@ -8,21 +8,21 @@ digest: - digesting 5µl of PCR fragments with the CAPS-marker specific restriction enzyme: -Marker Primer Sequence 5’-3’ Enzyme annealing temp (°C) EAM7 allele: fragment size (bp) eam7 allele: fragment size (bp) -M-13 F GTAATTGCCCTAGCGTTGGA MnlI 58°C 246, 98 328 +Marker Primer Sequence 5’-3’ Enzyme annealing temp (°C) EAM7 allele: fragment size (bp) eam7 allele: fragment size (bp) +M-13 F GTAATTGCCCTAGCGTTGGA MnlI 58°C 246, 98 328 R CAGAACCCGGGAATTAGGAT M-16 F AAAACGGTGGCATTAGCAAG HindIII 58°C 378 214, 168 R CGGAGGCATGATCAGGTATT -M-11 F AAAGGCCCACACTAGTGCAT BstUI 58°C 209, 128 337 +M-11 F AAAGGCCCACACTAGTGCAT BstUI 58°C 209, 128 337 R ACGCTCACTCCCTGCATCT M-39 F CTAAATTTCAGCCCCGAACA DdeI 55°C 237,100 334 R TGCCTGGACTTGTTCTGATG -M-PRR1 F GAGCATAGCATGGCACTTCA Hpy188I 58°C 224, 158 383 +M-PRR1 F GAGCATAGCATGGCACTTCA Hpy188I 58°C 224, 158 383 R GCCTGCCTAACAAACTGACC -lwd1-del F TACCATCCTCCCATTGCCTG - 60°C 199 167 +lwd1-del F TACCATCCTCCCATTGCCTG - 60°C 199 167 R ATTGGCTTACACTGCAGGGG -Marker Primer Sequence 5’-3’ Enzyme annealing temp (°C) ppd-H1 allele: fragment size (bp) Ppd-H1 allele: fragment size (bp) -M-PPDH11 F ACGTGAATGGTGGATCGGC BstUI 60°C 506 433, 73 +Marker Primer Sequence 5’-3’ Enzyme annealing temp (°C) ppd-H1 allele: fragment size (bp) Ppd-H1 allele: fragment size (bp) +M-PPDH11 F ACGTGAATGGTGGATCGGC BstUI 60°C 506 433, 73 R TATAGCTAGGTGCGTGGCG -- GitLab