diff --git a/studies/gene_expression_eam7_plants_diurnal/README.md b/studies/gene_expression_eam7_plants_diurnal/README.md index 63259b5119d24c3ee57720d2db797ef7084f7e18..0fb8f576b8bfffe2fea2a7687efdd9f3981c40d2 100644 --- a/studies/gene_expression_eam7_plants_diurnal/README.md +++ b/studies/gene_expression_eam7_plants_diurnal/README.md @@ -1,26 +1,20 @@ ## Test diurnal gene expression of circadian clock and flowering time genes in Bowman and Bowman-derived introgression lines. -2-week old plants from the genotypes BW, BW(Ppd-H1), BW(eam7), and BW(Ppd-H1,eam7) were sampled at Zeitgeber Time (ZT) 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22. +2-week old plants from the genotypes BW, BW(*Ppd-H1*), BW(*eam7*), and BW(*Ppd-H1,eam7*) were sampled at Zeitgeber Time (ZT) 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22. -Gene expression of CO1, ELF3, FT1, GI, LHY, LUX1, LWD1, PHYB, PHYC, Ppd-H1, PRR1, PRR59, PRR73, PRR95 and VRN1 were tested. +Gene expression of *CO1, ELF3, FT1, GI, LHY, LUX1, LWD1, PHYB, PHYC, Ppd-H1, PRR1, PRR59, PRR73, PRR95* and *VRN1* were tested. **Study overview:** - input: 2-weeks old BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD - - -> assay: leaf_sampling - output: frozen leaf tissue (3 biol. replicates) - - -> assay: RNA_extraction - output: RNA samples - - -> assay: cDNA_synthesis - output: cDNA samples - - -> assay: qRT-PCR - output: gene expression data (2 techn. replicates) - +```mermaid + flowchart TD; + A[Bowman and introgression lines] --> |leaf_sampling| B[frozen leaf tissues, 3 biol. replicates] + B --> |RNA_extraction| C[RNA samples] + C --> |cDNA_synthesis| D[cDNA samples] + D --> |qRT-PCR| E[gene expression data, 2 techn. replicates] +``` +**Publication:** Results can be found in Figure 3 and in Supplemental Figure S3E. \ No newline at end of file