From 6c594ebb0e5914e96918478e56009cdf4ac73a14 Mon Sep 17 00:00:00 2001 From: Gesa Helmsorig <gesa.helmsorig@hhu.de> Date: Wed, 13 Nov 2024 12:20:59 +0000 Subject: [PATCH] edited growth conditions / readme --- .../plant_material_growth_conditions.md | 22 +++++++++---------- .../README.md | 6 ++--- ...opment_plant_material_growth_conditions.md | 15 +++++++------ 3 files changed, 22 insertions(+), 21 deletions(-) diff --git a/studies/allelism_test/protocols/plant_material_growth_conditions.md b/studies/allelism_test/protocols/plant_material_growth_conditions.md index dcb4957..4a6c17e 100644 --- a/studies/allelism_test/protocols/plant_material_growth_conditions.md +++ b/studies/allelism_test/protocols/plant_material_growth_conditions.md @@ -1,33 +1,33 @@ -**Plant material and growth conditions** +## Plant material and growth conditions -**plant material** +**Plant material:** -species: Hordeum vulgare +Species: Hordeum vulgare -parent genotypes: +Parent genotypes: -- lwd1-26: lwd1 mutant in GP-fast background, truncated protein of 26 aa -- lwd1-390: lwd1 mutant in GP-fast background, truncated protein of 390 aa -- lwd1-402: lwd1 mutant in GP-fast background, truncated protein of 402 aa +- *lwd1-26*: lwd1 mutant in GP-fast background, truncated protein of 26 aa +- *lwd1-390*: lwd1 mutant in GP-fast background, truncated protein of 390 aa +- *lwd1-402*: lwd1 mutant in GP-fast background, truncated protein of 402 aa - GP-fast: Ppd-H1 introgression line in Golden Promise background, lwd1 mutant background, Gol et al. (2021) - BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011) - BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011) - BW(Ppd-H1, eam7): cross of the two introgression lines -**growth condtions** +**Growth condtions:** -parents: +Parents: - long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - 1.5 L pots (HerkuPlast Kubern GmbH, pot volume 75 cm^3) - Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) -- F1 crosses: +F1 crosses: - short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - 7x7x7.5 cm pots (pot volume: 367.5 cm^3) - Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) -**References** +**References:** Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110. 166249 diff --git a/studies/gene_expression_eam7_plants_development/README.md b/studies/gene_expression_eam7_plants_development/README.md index a58a0b3..76bfb17 100644 --- a/studies/gene_expression_eam7_plants_development/README.md +++ b/studies/gene_expression_eam7_plants_development/README.md @@ -1,13 +1,13 @@ -**Test diurnal gene expression of flowering time genes in Bowman and Bowman-derived introgression lines over development.** +## Test diurnal gene expression of flowering time genes in Bowman and Bowman-derived introgression lines over development. Genotypes BW, BW(Ppd-H1), BW(eam7), and BW(Ppd-H1,eam7) were grown under SD conditions. The youngest, fully elongated leaf was sampled at 19, 26, 33, 40, 47, and 59 DAE. Gene expression of FT1, FT2, FT3 and VRN1 were tested. -**study overview** +**Study overview:** -input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD + input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD -> assay: leaf_sampling output: frozen leaf tissue (3 biol. replicates) diff --git a/studies/gene_expression_eam7_plants_development/protocols/development_plant_material_growth_conditions.md b/studies/gene_expression_eam7_plants_development/protocols/development_plant_material_growth_conditions.md index 72e5312..6ae4939 100644 --- a/studies/gene_expression_eam7_plants_development/protocols/development_plant_material_growth_conditions.md +++ b/studies/gene_expression_eam7_plants_development/protocols/development_plant_material_growth_conditions.md @@ -1,9 +1,10 @@ -**eam7 developmental expression: plant material and growth conditions** +## *eam7* developmental expression: plant material and growth conditions -**plant material* +**Plant material** -species: Hordeum vulgare -genotypes: +Species: Hordeum vulgare + +Genotypes: - BW: Bowman, spring cultivar - BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011) @@ -11,20 +12,20 @@ genotypes: - BW(Ppd-H1, eam7): cross of the two introgression lines -**growth condtions** +**Growth condtions:** - short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - QuickPot E24/10 trays (HerkuPlast Kubern GmbH, pot volume 480 cm^3) - Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) -**sampling** +**Sampling:** - always sample youngest, fully elongated leaf - sample at ZT 9 (1h after lights turned off, as FT1 expression is highest) - sample genotypes at 19, 26, 33, 40, 47 and 59 DAE -**References** +**References:** Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.166249 -- GitLab