diff --git a/assays/RNA_extraction/protocols/ThermoFisher_DNAseI.pdf b/assays/RNA_extraction/protocols/ThermoFisher_DNAseI.pdf
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diff --git a/assays/RNA_extraction/protocols/trizol_RNA_extraction.txt b/assays/RNA_extraction/protocols/trizol_RNA_extraction.txt
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+TRIzol RNA extraction
+
+-	Place 2 3mm metal beads into 2.0 ml tube
+-	Collect samples directly into the tubes on liquid nitrogen
+-	Store samples at -80°C until needed
+
+-	Grind your samples 2 x 45sec at maximum frequency (30 Hertz) in TissueLyser (QIAGEN), use pre-cooled adapters
+-	Add 500 µL of TRIzol to the frozen samples, close tubes and mix vigorously until the sample is homogeneous
+-	Incubate the samples for 5 min at room temperature
+-	Briefly spin down the samples
+-	Add 100 µl of chloroform, close the tubes and shake tubes 15-20 sec vigorously by hand
+-	Incubate the samples for 3 min at room temperature
+-	Centrifuge the samples at 10.000 x g for 15 min at 4°C
+-	Transfer 150-300 µl of the clear, aqueous, upper phase to new tubes
+-	Add same volume of isopropyl alcohol, gently invert 8x, do not vortex
+-	Incubate the samples for 10 min at room temperature
+-	Centrifuge the samples at 10.000 x g for 10 min at 4°C
+-	Remove the supernatant
+-	Wash the pellet with 500 µl of 75% ethanol
+-	Centrifuge samples at 10.000 x g for 2 min at 4°C
+-	Remove the supernatant
+-	Dry the RNA pellet at least 10-30 min
+-	Add 60 µL of DEPC-water and incubate at 4°C over night
+
+-	Perform DNAse treatment (see ThermoFisher_DNAseI protocol)
+-	Store RNA at -80°C