diff --git a/studies/gene_expression_eam7_plants_development/README.md b/studies/gene_expression_eam7_plants_development/README.md index 76bfb17639ae1f654e02cd05d5d7fef554cefaf1..fcc6f0dd7d40b4f66749d4f968169e34afa93dcc 100644 --- a/studies/gene_expression_eam7_plants_development/README.md +++ b/studies/gene_expression_eam7_plants_development/README.md @@ -1,26 +1,19 @@ -## Test diurnal gene expression of flowering time genes in Bowman and Bowman-derived introgression lines over development. +## Test gene expression of flowering time genes in Bowman and Bowman-derived introgression lines over development. -Genotypes BW, BW(Ppd-H1), BW(eam7), and BW(Ppd-H1,eam7) were grown under SD conditions. The youngest, fully elongated leaf was sampled at 19, 26, 33, 40, 47, and 59 DAE. - -Gene expression of FT1, FT2, FT3 and VRN1 were tested. +Genotypes BW, BW(*Ppd-H1*, BW(*eam7*), and BW(*Ppd-H1,eam7*) were grown under SD conditions. The youngest, fully elongated leaf was sampled at 19, 26, 33, 40, 47, and 59 DAE. +Gene expression of *FT1*, *FT*2, *FT3* and *VRN1* were tested. **Study overview:** - input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD - - -> assay: leaf_sampling - output: frozen leaf tissue (3 biol. replicates) - - -> assay: RNA_extraction - output: RNA samples - - -> assay: cDNA_synthesis - output: cDNA samples - - -> assay: qRT-PCR - output: gene expression data (2 techn. replicates) - +```mermaid + flowchart TD; + A[Bowman and introgression lines] --> |leaf_sampling| B[frozen leaf tissues, 3 biol. replicates] + B --> |RNA_extraction| C[RNA samples] + C --> |cDNA_synthesis| D[cDNA samples] + D --> |qRT-PCR| E[gene expression data, 2 techn. replicates] +``` +**Publication:** Results can be found in Supplemental Figure S3A-D. \ No newline at end of file