diff --git a/assays/MSA_dissection/protocols/dissection_of_MSAs.txt b/assays/MSA_dissection/protocols/dissection_of_MSAs.md similarity index 83% rename from assays/MSA_dissection/protocols/dissection_of_MSAs.txt rename to assays/MSA_dissection/protocols/dissection_of_MSAs.md index b49b58e714fd1a359937982b3e1c93a1c3cc058a..eccde7f272f1464cc793c8e8d26a7c866ad11dc5 100644 --- a/assays/MSA_dissection/protocols/dissection_of_MSAs.txt +++ b/assays/MSA_dissection/protocols/dissection_of_MSAs.md @@ -1,6 +1,10 @@ +## Dissection of main shoot apices (MSAs) of barley plants + +**Growth:** - per genotype: choose approx. 4 random plants every 6-8 days (SD) or every 2-10 days (LD) - start 5 days after emergence (DAE) +**Dissection:** - remove the leaves surrounding the main shoot apex (MSA) with a blade - image MSA with the stereo microscope Nikon SMZ18 with a Nikon DS-Fi2 camera - score developmental stage according to Waddington et al. (1983)