From 738df60a21542fc36299a5120f4f2eb8f950e224 Mon Sep 17 00:00:00 2001
From: Gesa Helmsorig <gesa.helmsorig@hhu.de>
Date: Wed, 13 Nov 2024 12:56:07 +0000
Subject: [PATCH] Update 2 files
- /assays/MSA_dissection/protocols/dissection_of_MSAs.txt
- /assays/MSA_dissection/protocols/dissection_of_MSAs.md
---
.../{dissection_of_MSAs.txt => dissection_of_MSAs.md} | 4 ++++
1 file changed, 4 insertions(+)
rename assays/MSA_dissection/protocols/{dissection_of_MSAs.txt => dissection_of_MSAs.md} (83%)
diff --git a/assays/MSA_dissection/protocols/dissection_of_MSAs.txt b/assays/MSA_dissection/protocols/dissection_of_MSAs.md
similarity index 83%
rename from assays/MSA_dissection/protocols/dissection_of_MSAs.txt
rename to assays/MSA_dissection/protocols/dissection_of_MSAs.md
index b49b58e..eccde7f 100644
--- a/assays/MSA_dissection/protocols/dissection_of_MSAs.txt
+++ b/assays/MSA_dissection/protocols/dissection_of_MSAs.md
@@ -1,6 +1,10 @@
+## Dissection of main shoot apices (MSAs) of barley plants
+
+**Growth:**
- per genotype: choose approx. 4 random plants every 6-8 days (SD) or every 2-10 days (LD)
- start 5 days after emergence (DAE)
+**Dissection:**
- remove the leaves surrounding the main shoot apex (MSA) with a blade
- image MSA with the stereo microscope Nikon SMZ18 with a Nikon DS-Fi2 camera
- score developmental stage according to Waddington et al. (1983)
--
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