diff --git a/studies/phenotyping_eam7_plants/README.md b/studies/phenotyping_eam7_plants/README.md index 1278a5e3421ceb12aab5014a7fc5ff5482798f9f..980ecdc7789f3b0601ac030e0b83dc32ea202767 100644 --- a/studies/phenotyping_eam7_plants/README.md +++ b/studies/phenotyping_eam7_plants/README.md @@ -1,27 +1,30 @@ -**Phenotype *eam7* plants under SD and LD conditions** +## Phenotype *eam7* plants under SD and LD conditions Grow plants under short-day (SD) and long-day (LD) conditions. Score flowering time and phenotypic traits at flowering and at harvest when plants are fully dry. -**study overview** +**Study overview:** -input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD and LD + input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD and LD -> assay: plant_phenotyping output: SD plants - flowering time (in DAE) - - at flowering: plant height, leaf number, tiller number, flag leaf length/width - - at harvest (on main culm): spike length, floret and grain number, spike fertility + - at flowering: plant height, leaf number, tiller number, + flag leaf length/width + - at harvest (on main culm): spike length, floret and grain number, + spike fertility LD plants - flowering time (in DAE) - at flowering: plant height, leaf number, tiller number - - at harvest (on main culm): spike length, floret and grain number, spike fertility + - at harvest (on main culm): spike length, floret and grain number, + spike fertility Results can be found in Figure 1A and B, Figure 2, and Supplemental Figure S2. -**References** +**References:** Waddington SR, Cartwright PM, Wall PC. A quantitative scale of spike initial and pistil development in barley and wheat. Ann Bot. 1983:51(1):119–130. https://doi.org/10.1093/oxfordjournals.aob.a086434 diff --git a/studies/phenotyping_eam7_plants/protocols/plant_material_growth_conditions.md b/studies/phenotyping_eam7_plants/protocols/plant_material_growth_conditions.md index 26fd61e61fe63c2d21e1a8d76d5d0a3356ee55a2..0ead6399f2288df4e85e459606593a3ae06bddcd 100644 --- a/studies/phenotyping_eam7_plants/protocols/plant_material_growth_conditions.md +++ b/studies/phenotyping_eam7_plants/protocols/plant_material_growth_conditions.md @@ -1,24 +1,23 @@ -**Plant material and growth conditions** +## Plant material and growth conditions -**plant material** +**Plant material:** -species: Hordeum vulgare +Species: *Hordeum vulgare* -genotypes: +Genotypes: - BW: Bowman, spring cultivar - BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011) - BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011) - BW(Ppd-H1, eam7): cross of the two introgression lines -**growth condtions** +**Growth condtions:** - short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - - QuickPot E24/10 trays (HerkuPlast Kubern GmbH, pot volume 480 cm^3) - Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) -**References** +**References:** Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.166249