From 7a043b18e38fc2e2b4089daf46256aefb45a4846 Mon Sep 17 00:00:00 2001
From: Gesa Helmsorig <gesa.helmsorig@hhu.de>
Date: Wed, 13 Nov 2024 12:30:21 +0000
Subject: [PATCH] Update 2 files

- /studies/phenotyping_eam7_plants/protocols/plant_material_growth_conditions.md
- /studies/phenotyping_eam7_plants/README.md
---
 studies/phenotyping_eam7_plants/README.md       | 17 ++++++++++-------
 .../plant_material_growth_conditions.md         | 13 ++++++-------
 2 files changed, 16 insertions(+), 14 deletions(-)

diff --git a/studies/phenotyping_eam7_plants/README.md b/studies/phenotyping_eam7_plants/README.md
index 1278a5e..980ecdc 100644
--- a/studies/phenotyping_eam7_plants/README.md
+++ b/studies/phenotyping_eam7_plants/README.md
@@ -1,27 +1,30 @@
-**Phenotype *eam7* plants under SD and LD conditions**
+## Phenotype *eam7* plants under SD and LD conditions
 
 Grow plants under short-day (SD) and long-day (LD) conditions. Score flowering time and phenotypic traits at flowering and at harvest when plants are fully dry.
 
-**study overview**
+**Study overview:**
 
-input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD and LD
+	input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD and LD
 
 	-> assay: plant_phenotyping
 		output: 
 			SD plants 
 				- flowering time (in DAE)
-				- at flowering: plant height, leaf number, tiller number, flag leaf length/width
-				- at harvest (on main culm): spike length, floret and grain number, spike fertility
+				- at flowering: plant height, leaf number, tiller number, 
+				flag leaf length/width
+				- at harvest (on main culm): spike length, floret and grain number, 
+				spike fertility
 			LD plants 
 				- flowering time (in DAE)
 				- at flowering: plant height, leaf number, tiller number
-				- at harvest (on main culm): spike length, floret and grain number, spike fertility
+				- at harvest (on main culm): spike length, floret and grain number, 
+				spike fertility
 						
 				
 
 Results can be found in Figure 1A and B, Figure 2, and Supplemental Figure S2.
 
 
-**References**
+**References:**
 
 Waddington SR, Cartwright PM, Wall PC. A quantitative scale of spike initial and pistil development in barley and wheat. Ann Bot. 1983:51(1):119–130. https://doi.org/10.1093/oxfordjournals.aob.a086434
diff --git a/studies/phenotyping_eam7_plants/protocols/plant_material_growth_conditions.md b/studies/phenotyping_eam7_plants/protocols/plant_material_growth_conditions.md
index 26fd61e..0ead639 100644
--- a/studies/phenotyping_eam7_plants/protocols/plant_material_growth_conditions.md
+++ b/studies/phenotyping_eam7_plants/protocols/plant_material_growth_conditions.md
@@ -1,24 +1,23 @@
-**Plant material and growth conditions**
+## Plant material and growth conditions
 
-**plant material**
+**Plant material:**
 
-species: Hordeum vulgare
+Species: *Hordeum vulgare*
 
-genotypes: 
+Genotypes: 
 - BW:  Bowman, spring cultivar
 - BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011)
 - BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011)
 - BW(Ppd-H1, eam7): cross of the two introgression lines
 
-**growth condtions**
+**Growth condtions:**
 
 - short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
 - long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
-
 - QuickPot E24/10 trays (HerkuPlast Kubern GmbH, pot volume 480 cm^3)
 - Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
 
 
-**References**
+**References:**
 
 Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.166249
-- 
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