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diff --git a/assays/MSA_dissection/protocols/dissection_of_MSAs.txt b/assays/MSA_dissection/protocols/dissection_of_MSAs.txt
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+- per genotype: choose approx. 4 random plants every 6-8 days (SD) or every 2-10 days (LD)
+- start 5 days after emergence (DAE)
+
+- remove the leaves surrounding the main shoot apex (MSA) with a blade
+- image MSA with the stereo microscope Nikon SMZ18 with a Nikon DS-Fi2 camera
+- score developmental stage according to Waddington et al. (1983)
+- count spikelet meristems (include those that have developet into floret meristems or florets) on the MSA
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diff --git a/studies/MSA_dissection/README.md b/studies/MSA_dissection/README.md
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+Scoring of the development according to:
+
+Waddington SR, Cartwright PM, Wall PC. A quantitative scale of spike initial and pistil development in barley and wheat. Ann Bot. 1983:51(1):119–130. https://doi.org/10.1093/oxfordjournals.aob.a086434
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diff --git a/studies/MSA_dissection/protocols/plant_material_growth_conditions.md b/studies/MSA_dissection/protocols/plant_material_growth_conditions.md
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-**Plant material and growth conditions**
-
-**plant material**
-
-species: Hordeum vulgare
-
-genotypes: 
-- BW:  Bowman, spring cultivar
-- BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011)
-- BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011)
-- BW(Ppd-H1, eam7): cross of the two introgression lines
-
-**growth condtions**
-
-- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
-- long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
-
-- QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3)
-- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
-
-
-**plants**
-- SD: sample names include "SD"
-- LD: sample names include "LD"
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+**Plant material and growth conditions**
+
+**plant material**
+
+species: Hordeum vulgare
+
+genotypes: 
+- BW:  Bowman, spring cultivar
+- BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011)
+- BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011)
+- BW(Ppd-H1, eam7): cross of the two introgression lines
+
+**growth condtions**
+
+- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
+- long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
+
+- QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3)
+- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
+
+
+**plants**
+- SD: plant 1 - 204
+- LD: plant 205 - 342
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