diff --git a/assays/MSA_dissection/README.md b/assays/MSA_dissection/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/MSA_dissection/dataset/.gitkeep b/assays/MSA_dissection/dataset/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/MSA_dissection/dataset/LD_dissection_results.xlsx b/assays/MSA_dissection/dataset/LD_dissection_results.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..64dc4cf7dee6f3f8928601e992b509edbab94304 Binary files /dev/null and b/assays/MSA_dissection/dataset/LD_dissection_results.xlsx differ diff --git a/assays/MSA_dissection/dataset/SD_dissection_results.xlsx b/assays/MSA_dissection/dataset/SD_dissection_results.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..c49bb87040186720fe0d2dacf60544887e0f70ed Binary files /dev/null and b/assays/MSA_dissection/dataset/SD_dissection_results.xlsx differ diff --git a/assays/MSA_dissection/isa.assay.xlsx b/assays/MSA_dissection/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..4e0133763d8aa3a64189bd27caed8a34646ff8ad Binary files /dev/null and b/assays/MSA_dissection/isa.assay.xlsx differ diff --git a/assays/MSA_dissection/protocols/.gitkeep b/assays/MSA_dissection/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/MSA_dissection/protocols/dissection_of_MSAs.txt b/assays/MSA_dissection/protocols/dissection_of_MSAs.txt new file mode 100644 index 0000000000000000000000000000000000000000..b49b58e714fd1a359937982b3e1c93a1c3cc058a --- /dev/null +++ b/assays/MSA_dissection/protocols/dissection_of_MSAs.txt @@ -0,0 +1,7 @@ +- per genotype: choose approx. 4 random plants every 6-8 days (SD) or every 2-10 days (LD) +- start 5 days after emergence (DAE) + +- remove the leaves surrounding the main shoot apex (MSA) with a blade +- image MSA with the stereo microscope Nikon SMZ18 with a Nikon DS-Fi2 camera +- score developmental stage according to Waddington et al. (1983) +- count spikelet meristems (include those that have developet into floret meristems or florets) on the MSA \ No newline at end of file diff --git a/studies/MSA_dissection/README.md b/studies/MSA_dissection/README.md index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..7c3f78dd39ef6f91cf32b48b9da1bbab6d616576 100644 --- a/studies/MSA_dissection/README.md +++ b/studies/MSA_dissection/README.md @@ -0,0 +1,3 @@ +Scoring of the development according to: + +Waddington SR, Cartwright PM, Wall PC. A quantitative scale of spike initial and pistil development in barley and wheat. Ann Bot. 1983:51(1):119–130. https://doi.org/10.1093/oxfordjournals.aob.a086434 \ No newline at end of file diff --git a/studies/MSA_dissection/isa.study.xlsx b/studies/MSA_dissection/isa.study.xlsx index b31a631cad21d62fa566750bd05a70ad41cadf97..f01de988dbf143579127bad54ffd4b1689d5abd7 100644 Binary files a/studies/MSA_dissection/isa.study.xlsx and b/studies/MSA_dissection/isa.study.xlsx differ diff --git a/studies/MSA_dissection/protocols/plant_material_growth_conditions.md b/studies/MSA_dissection/protocols/plant_material_growth_conditions.md index 5c4ad3a39e908b1fb5fe4c3ba80222d6fc3d2bd0..944ca4979a0de5aaaecfcf41366613cacf96d0bb 100644 --- a/studies/MSA_dissection/protocols/plant_material_growth_conditions.md +++ b/studies/MSA_dissection/protocols/plant_material_growth_conditions.md @@ -1,24 +1,24 @@ -**Plant material and growth conditions** - -**plant material** - -species: Hordeum vulgare - -genotypes: -- BW: Bowman, spring cultivar -- BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011) -- BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011) -- BW(Ppd-H1, eam7): cross of the two introgression lines - -**growth condtions** - -- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) -- long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - -- QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3) -- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) - - -**plants** -- SD: sample names include "SD" -- LD: sample names include "LD" \ No newline at end of file +**Plant material and growth conditions** + +**plant material** + +species: Hordeum vulgare + +genotypes: +- BW: Bowman, spring cultivar +- BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011) +- BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011) +- BW(Ppd-H1, eam7): cross of the two introgression lines + +**growth condtions** + +- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) +- long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR) + +- QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3) +- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) + + +**plants** +- SD: plant 1 - 204 +- LD: plant 205 - 342 \ No newline at end of file