diff --git a/assays/complementation_crosses/protocols/crossing_plants.md b/assays/complementation_crosses/protocols/crossing_plants.md index b090a5eaae8dcffaacb3650b63cc5c1021045968..59222e7927c797078be387fcfa9d618554f9e6b8 100644 --- a/assays/complementation_crosses/protocols/crossing_plants.md +++ b/assays/complementation_crosses/protocols/crossing_plants.md @@ -1,9 +1,10 @@ -**crossing of plants** +## Crossing of plants +**Growth:** - grow parent plants in LD conditions - if parental plants have very different flowering times: sow the faster one with a bit of delay) - +**Crossing:** - emasculate florets from mother plants before pollen are shed (about Waddington stage 8.5 - 9.0) - pollinate florets with pollen from father plants, pollinate all florets from one spike with the same father: this is one cross - cover the spike with a paper bag, count later how many of the pollinated florets develop into seeds