From 9b14919412da8fce0bcd8c6297059a5a4b53134d Mon Sep 17 00:00:00 2001 From: Gesa Helmsorig <gesa.helmsorig@hhu.de> Date: Wed, 13 Nov 2024 12:52:18 +0000 Subject: [PATCH] Update file crossing_plants.md --- assays/complementation_crosses/protocols/crossing_plants.md | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/assays/complementation_crosses/protocols/crossing_plants.md b/assays/complementation_crosses/protocols/crossing_plants.md index b090a5e..59222e7 100644 --- a/assays/complementation_crosses/protocols/crossing_plants.md +++ b/assays/complementation_crosses/protocols/crossing_plants.md @@ -1,9 +1,10 @@ -**crossing of plants** +## Crossing of plants +**Growth:** - grow parent plants in LD conditions - if parental plants have very different flowering times: sow the faster one with a bit of delay) - +**Crossing:** - emasculate florets from mother plants before pollen are shed (about Waddington stage 8.5 - 9.0) - pollinate florets with pollen from father plants, pollinate all florets from one spike with the same father: this is one cross - cover the spike with a paper bag, count later how many of the pollinated florets develop into seeds -- GitLab